内毒素发热时家兔不同脑区组织,脑脊液及血浆中CAMP含量变化的比较

为验证内毒素(ET)发热时脑脊液(csf)cAMP含量增高的来源以及cAMP的中枢发热介质作用,本文应用ET复制发热模型,同时测定不同脑区组织中cAMP含量并与csf,血浆中cAMP含量进行同步比较。结果发现:ET发热时,丘脑下部组织中cAMP含量和csf cAMP含量均明显增加,与发热的体温变化呈明显正相关关系,而脑干、脑皮质组织中cAMP含量以及血浆中cAMP含量则变化无规律性。表明:ET发热时csf cAMP含量增加可以反映出丘脑下部组织中cAMP含量变化。其主要来源于丘脑下部组织。脑内cAMP作用的相对特异性部位亦在丘脑下部。

The cAMP levels in the tissues of different brain regions in comparison with that of csf, plasma during ET-induced fever in rabbits

In order to determine whether the cAMP is a central madiator and participates in the mechanism of the ET-induced fever, in the following experiments, we have compared the cAMP levels in csf, plasma to the cAMP levels in tissues of hypothalamus, brain-stem and brain-cortex during the ET-induced fever. The results obtained demonstrate: the cAMP Ievels in tissues of hypothalamus and in csf are increased markedly during the fever Compared with the normal control. These changes of the cAMP levels are paralleled and correlated apparently with the body temperature. However, there isn't any obvious change of the cAMP levels in the tissues of brain-stem, brain-cortex and in plasma during the fever. This demonstrats that the cAMP increased in csf may indirectly reflect cAMP level in hypothalamus during the fever in rabbits and the main source of cAMP increased in csf may be synthesized and released by hypothalamus.