不同来源赶黄草的ISSR分析

目的对不同来源的赶黄草进行遗传多样性分析,为进一步开发利用和保护赶黄草药用资源提供理论基础。方法利用ISSR分子标记方法对7个不同来源110个赶黄草样品进行DNA分子水平的评价,采用PAUP 4.0b软件处理进行聚类分析。结果筛选出19个ISSR引物,利用其中3条引物进行了不同来源赶黄草样品的ISSR-PCR分析,共获得34条多态性条带,多态位点百分率为100%,同时进行了居群间遗传多样性差异分析。结论赶黄草四川居群与其他两个居群间存在着一定的分化。本方法在评价赶黄草遗传多样性、亲缘关系、品种鉴别、遗传进化等方面有很好的适用性,可为赶黄草及其近缘属植物的系统学研究提供更多信息。

Analysis on Genetic Diversity of Penthorum chinense Pursh from Separate Sources Based on ISSR Markers

Objectives Genetic diversity analysis of Penthorum chinense Pursh was performed in order to pro- vide the theoretical basis of the exploitation of the medical resources of Penthorum chinense Pursh. Methods ISSR molecular markers were used to analyze the genetic diversity of Penthorum chinense Pursh from separate sources. PAUP 4.0b software was used for the cluster analysis. Results 3 primers of 19 ISSR primers screened out were used in ISSR- PCR, and 34 polymorphic bands obtained were representative of 100% polymorphism. Conclusion There were differentiations between Sichuan population and the others Penthorum chinense Pursh tested. The method was applicable for evaluating genetic diversity, affiliation, variety appreciation and genetic evolution of Penthorum chinense Pursh.