Enhanced Induction by X-irradiation of DNA Double-strand Breakage in Mitotic as Compared with S-phase V79 Cells |
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| Authors: | Ian R. Radford Sam Broadhurst |
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| Affiliation: | Biological Research Unit, Cancer Institute, 481 Little Lonsdale Street, Melbourne, Victoria, 3000, Australia |
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| Abstract: | SummaryA marked enhancement of X-ray-induced DNA double-strand breakage was demonstrated for mitotic Chinese hamster (V79) cells compared with S-phase cells or asynchronous cultures, which can explain the high radiosensitivity of mitotic cells. However, mitotic cells required twice the level of DNA double-strand breakage to produce a lethal lesion of that required for S-phase or asynchronous cells, suggesting that repair in mitotic cells is less error prone. |
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| Keywords: | DNA double-strand breaks neutral filter elution X-irradiation cell-cycle dependent killing nocodazole |
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