帕米膦酸钠对大鼠正畸牙移动过程中破牙骨质细胞及破骨细胞分化因子表达的影响

目的:研究帕米膦酸钠对大鼠正畸牙移动过程中牙周组织压力侧破牙骨质细胞及破骨细胞分化因子(ODF)表达的影响.方法:选择24只6周龄SPF级健康雌性Wistar大鼠,建立正畸牙移动动物模型,每只大鼠上颌分实验侧和对照侧,于安装矫治器前3d,于实验侧大鼠第一磨牙近中腭侧黏骨膜下注射帕米膦酸钠50 μL,对照侧注射0.9％生理盐水50 μL,每3d注射1次.于正畸加力3、7、14 d时分批处死8只大鼠,制作牙周组织切片,观察破牙骨质细胞数量,免疫组化观察ODF的表达情况.采用PASW Statistics 18软件包对实验数据进行统计学处理.结果:实验侧在3、7、14d时,第一磨牙压力侧破牙骨质细胞的数目均少于对照侧,其中,7、14d时两侧差异显著(P＜0.05);实验侧在3、7、14d时,第一磨牙压力侧ODF阳性表达均低于对照侧,其中,7、14 d时两侧差异显著(P＜0.05).结论:局部注射二膦酸盐帕米膦酸钠能够减少大鼠正畸牙移动过程中牙周组织压力侧破牙骨质细胞的数量及ODF的阳性表达.

Effect of pamidronate on cementoclast and osteoclast differentiation factor expression during orthodontic tooth movement in rats

PURPOSE: To investigate the influence of pamidronate on cementoclast and osteoclast differentiation factor(ODF) expression during orthodontic tooth movement in rats.METHODS: 24 female,6-week-old SPF Wistar rats were selected and the animal models for orthodontic tooth movement were established.Each rat ’s maxillary was divided into experimental side and control side.Pamidronate(50 μL) was injected into the sub-periosteum area adjacent to the left upper first molar(experimental side) every 3 days during the experiment,while 0.9%NS(50 μL) was injected into the corresponding area of the control side.The rats were executed in batch on the 3rd,7th and 14th day respectively during the orthodontic treatment for detecting the amount of cementoclast and the expression of ODF in the periodontal tissues. All statistical analysis was performed using PASW Statistics 18 software package.RESULTS: The amount of cementoclasts was significantly less in the experimental side than that in the control side on the 7th and 14th days(P< 0.05).The expression of ODF was significantly less in the experimental side than that in the control side on the 7th and 14th days(P<0.05).CONCLUSIONS: Local injection of pamidronate can reduce the number of cementoclast and the expression of ODF.