白藜芦醇对黑色素瘤细胞增殖的抑制作用

目的:探讨白藜芦醇对黑色素瘤细胞WM239增殖、细胞周期及凋亡的影响。方法:MTT法检测白藜芦醇对WM239细胞增殖的影响;流式细胞术检测白藜芦醇对WM239细胞周期及凋亡的影响。结果:12.5～200μmol/L的白藜芦醇作用于WM239细胞48h和72h后,对其增殖具有一定的抑制作用,作用72h时,浓度>50μmol/L其抑制率>50%,且抑制程度与药物浓度呈正相关。其中0、25、50和100μmol/L药物与WM239细胞作用48h后,凋亡率分别为0.1%、9.7%、8.9%和19.3%,处理组与未处理组相比细胞凋亡明显增加,G0/G1期细胞分别为71.9%、77.3%、81.0%和83.7%,S期分别为21.5%、17.9%、16.0%和14.6%,G2/M期为6.5%、4.8%、2.9%和1.8%,细胞周期阻滞于G1期,S期和G2期细胞比例下降,剂量依赖关系明显。结论:白藜芦醇可以抑制WM239细胞的增殖,引起细胞周期改变,凋亡细胞明显增加。

Inhibition of resveratrol on the proliferation of melanoma cells

OBJECTIVE: To explore the influence of resveratrol on the growth,cell cycle and apoptosis of melanoma cell lines WM239.METHODS:The effects of resveratrol on the proliferation of WM239 cells were analyzed with MTT assay.The cell cycle and apoptosis was analyzed using flow cytometry with Annexin-V and PI staining.RESULTS: The proliferation of WM239 was inhibited when the cell line was exposed to 12.5-200 μmol/L resveratrol for 48 h and 72 h.The inhibition rate was higher than 50% when the cell line was exposed to above 50 μmol/L resveratrol for 72 h.The inhibition was positively correlated to drug concentration.Exposed to 0,25,50 and 100 μmol/L resveratrol for 48 h,the apoptosis rate were 0.1%,9.7%,8.9% and 19.3%.G0/G1 phase cells were 71.9%,77.3%,81.0% and 83.7% respectively.S phase cells were 21.5%,17.9%,16.0% and 14.6%.G2/M phase cells were 6.5%,4.8%,2.9% and 1.8%.The cell cycle was blocked in G1 phase.The cells of S and G2 phase decreased in a dose-dependent manner.CONCLUSION:Resveratrol could inhibit the proliferation of WM239 cells,affect the cell cycle and cause the apoptosis of WM239 cells.