| 反相高效液相色谱法快速测定3种抗癫痫药物血药浓度 |
| |
| 引用本文： | 孙增先,周金玉,张骞峰,陶爱华.反相高效液相色谱法快速测定3种抗癫痫药物血药浓度[J].中国现代应用药学,2004,21(4):316-318. |
| |
| 作者姓名： | 孙增先 周金玉 张骞峰 陶爱华 |
| |
| 作者单位： | 连云港市第一人民医院,江苏,连云港,222002 |
| |
| 摘 要： | 目的建立快速反相高效液相色谱法测定3种抗癫痫药血药浓度.方法选择醋酸乙酯为萃取溶剂;采用Nova-PakC18柱(4μm,3.9mm×150mm)为分析柱;0.01moL/L磷酸盐缓冲液(pH3.5)-甲醇(50:50)为流动相;检测波长210nm;流速1mL/min.结果该色谱条件下3种药物分离良好,苯巴比妥、苯妥英钠、卡马西平线性范围为1～100,1～40,1～20μg·mL-1,平均回收率分别为99.8%,99.6%,100.6%.日内、日间RSD均小于6%;用于229例临床患者监测,在服用苯妥英钠的患者中有56.1%的患者血药浓度低于有效浓度,20.1%高于有效浓度,并出现中毒症状.结论本法快速(5min)、简便、结果准确,更适合临床实际应用.
|
| 关 键 词： | 反相高效液相色谱法 苯巴比妥 苯妥英钠 卡马西平 血药浓度 |
| 文章编号： | 1007-7693(2004)04-0316-03 |
Rapid determination of three antiepileptic drugs in serum by reversed phase high performance liquid chromatography |
| |
| SUN Zeng-xian,ZHOU Jin-yu,ZHANG Qian-feng,TAO Ai-hua.Rapid determination of three antiepileptic drugs in serum by reversed phase high performance liquid chromatography[J].The Chinese Journal of Modern Applied Pharmacy,2004,21(4):316-318. |
| |
| Authors: | SUN Zeng-xian ZHOU Jin-yu ZHANG Qian-feng TAO Ai-hua |
| |
| Abstract: | OBJECTIVE To establish a rapid analytical method for the determination of phenobartial(PB),phenytoin(DPH) and carbamazepine(CBZ) in serum by reversed phase high performance liquid chromatography(RP-HPLC). METHOD Ethyl acetate was selected as extract sol |
| |
| Keywords: | reversed phase high performance liquid chromatography phenobartial phenytoin carbamazepine serum drug concentration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国现代应用药学》浏览原始摘要信息 |
| 点击此处可从《中国现代应用药学》下载免费的PDF全文 |
|
