魔芋多糖对小鼠瘦素和Na~+-K~+-ATP酶水平的影响

目的探讨魔芋多糖对高脂饮食小鼠血清瘦素和小肠粘膜Na+-K+-ATP酶水平的影响。方法将实验动物分为正常对照组、高脂对照组和高、中、低剂量魔芋多糖与高脂联合处理组共5个组,连续喂饲20天,用ELISA法测定血清瘦素水平,分光光度法测定小肠粘膜Na+-K+-ATP酶活性以及体重、体脂、血糖等指标。结果第10天高脂对照组的体重和餐后血糖为(31.3±2.11)g和(7.5±1.15)mmol/L,魔芋多糖高剂量组则为(28.0±2.06)g和(4.8±0.73)mmol/L。第20天高脂对照组的血清瘦素浓度和小肠粘膜Na+-K+与高脂联合处理-ATP酶活性为(1078.5±61.69)pg/ml和(16.2±1.48)μmolPi/(mg pro·h),魔芋多糖高剂量与高脂联合处理组则为(820.5±58.52)pg/ml和(11.2±1.10)μmolPi/(mgpro·h)。两组间各项指标差别均有显著性意义(P<0.05)。结论魔芋多糖能够降低高脂饮食小鼠餐后血糖、血清瘦素水平和小肠粘膜Na+-K+-ATP酶活性,抑制体重和体脂的增加。

Effect of konjac polysaccharide on levels of leptin and Na+-K+-atpase of mice treated with high fat

Objective To investigate the effect of Konjac polysaccharide on serum leptin and intestinal mucosa Na~+-K~+-ATPase activity of mice treated with high fat. Methods Mice were divided into normal control, high-fat control, and high, middle, low dose of konjac polysaccharide combining high fat groups. The mice were feed for twenty days.The levels of serum leptin were determined by ELISA method, and intestinal mucosa Na~+-K~+-ATPase activities were determined by spectrophotometry. The body weight, adipose tissue and blood glucose were also examined. Results At the 10th day, body weight and postprandial blood glucose of high-fat control group were (31.3±2.11)g and (7.5±1.15)mmol/L, those of high dose konjac polysaccharide combining high fat group were(28.0±2.06)g and (4.8±0.73)mmol/L. At the 20th day, concentrations of serum leptin and Na~+-K~+-ATPase activities of high-fat control group were (1078.5±61.69)pg/ml and (16.2±1.48) μmol Pi/(mg pro·h), those of high dose konjac polysaccharide combining high fat group were (820.5±58.52)pg/ml and (11.2±1.10) μmol Pi/(mg pro·h). All differences between two groups were significant (P<0.05). Conclusion Konjac polysaccharide could decrease level of body weight, postprandial blood glucose, serum leptin and intestinal mucosa Na~+-K~+-ATPase activity of mice treated with high fat.