人子宫内膜异位症裸鼠模型的建立

目的：建立人子宫内膜异位症裸鼠模型。方法：4只参加实验的裸鼠分戍2组，每组2只，分别用注射人子宫内膜碎屑液入裸鼠腹腔和手术将人子宫内膜组织块贴于裸鼠卵巢的方法建立模型，观察移植内膜的组织学、超微结构情况。结果：手术组人子宫内膜异位症裸鼠模型成功建立，光镜下见内膜细胞及间质生长，电镜下见丰富粗面内质网，线粒体丰富，纤维增生，提示内膜细胞生长活跃；注射组未能成功建立模型。结论：外科手术移植法是建立人子宫内膜异位症裸鼠模型有效的方法。

Establishment of Human Endometriosis Model in Nude Mice

Objective; To establish the human endometriosis model. Method; Four nude mice were divided into 2 groups with 2 in each. Group A; a minilaparotomy was performed to place fresh human endometrial samples on the ovary of nude mice. Group B: Inject the human endometrial chipping to the peritonea] cavity of nude mice. Sixteen days later, remove the transplants to be examined by microscopy. Results:The human endometriosis model was successful established in group A. Under the microscope we can see the glands of endometrium. By electron microscopy we can see large number of mitochondria and rough endoplasmic reticulum. No endometrium was found in Group B. Conclusion; Surgical transplantation is effective method to establish the human endometriosis model in nude mice.