基质金属蛋白酶MMP-2 MMP-9及其抑制因子TIMP-1TIMP-2在子宫内膜异位症中的表达及意义

目的　研究基质金属蛋白酶MMP 2、9和组织抑制因子TIMP 1、2在子宫内膜异位症中的表达及意义。方法　 2 0 0 0年 3月至 2 0 0 2年 4月应用免疫组化S P法检测卵巢巧克力囊肿 4 5例的异位内膜和其中 2 0例在位内膜 ,以及 2 2例正常子宫内膜中MMP 2、9及TIMP 1、2的表达。结果　异位内膜组织中MMP 2、9和TIMP 1、2的表达均显著高于在位内膜和正常内膜 (P <0 0 1)。在位内膜和正常子宫内膜组织细胞中的表达率比较 ,差异无显著性 (P >0 0 5 )。子宫内膜异位症组织中MMP 2、9的表达与侵袭程度正相关。TIMP 1、2的表达则与侵袭程度呈负相关。结论　MMP 2、9是检测子宫内膜异位症较好的分子标志 ,人工诱导TIMP 1、2或阻断MMP 2、9的表达可能抑制内异症的发生发展 ,有望成为子宫内膜异位症治疗新的辅助手段

Expression and significance of matrix metalloproteinase-2,9 and tissue inhibitor of metalloproteinase-1,2 protein in endometriosis

Objective To study the clinical significance and expression of matrix metalloproteinase-2,9(MMP-2,9) and tissue inhibitor of metalloproteinase-1,2(TIMP-1,2) protein in endometriosis.Methods Immunohistochemical S-P method was employed to detect the expression of MMP-2,9 and TIMP-1,2 in the ectopic endometrium of the patients with ovarian endometriosis (45 cases),the eutopic endometrium of the patients with endometriosis(20 cases) and the normal controls(22 cases).Results The expression levels of MMP-2,9 and TIMP-1,2 in the normal endometrium and the eutopic endometrium were lower than those in endometriosis (P<0.01).There was no obvious difference between the expression level of eutopic endometrium and that of normal endometrium (P>0.05).The expressions of MMP-2,9 in endometriotic tissues had positive correlation with the invasiveness of endometriosis.The expressions of TIMP-1,2 had negative correlation with the invasiveness of endometriosis.Conclusion MMP-2,9 are better molecule indicators for detecting endometriosis.Artificially induced expression of TIMP-1,2 or interrupting the expression of MMP-2,9 probably inhibits the pathogenesis of endometriosis.This study may provide a new auxiliary way for therapy of endometriosis.