土槿乙酸衍生物抗真菌活性研究

【目的】对14种土槿乙酸衍生物进行体外抗真菌试验，测定其最低抑菌浓度（MIC80）值。【方法】采用微量液基稀释法进行药敏试验。【结果】除衍生物PB-9外，其余13种衍生物抗真菌活性均优于或相当于土槿乙酸。衍生物PB-3抗真菌作用最强，对5株真菌的MIC80在8～64μg／ml之间，其对絮状表皮癣菌抑制作用与二性霉素B相同。【结论】某些土槿乙酸衍生物具有更强的抗真菌活性，有进一步开发价值。

Study on the antifungal activity of the derivatives of pseudolaric acid B

[Objective] To test the antifungal activity of fourteen derivatives of pseudolaric acid B in vitro and measure the minimal inhibitory concentration （ MIC80 ）. [Methods] The drug susceptibility test was employed by broth microdilution procedure proposed by the National Committee for Clinical Laboratory Standards （NCCLS）. [Results] Except for derivative PB-9, the antifungal activities of the other thirteen derivatives exceeded pseudolaric acid B or were equal to it. The derivative PB-3 showed the strongest antifungal activity and the MIC80 for five tested fungi were between 8 μg/ml to 64 μg/ml. The inhibitory effect on Epidermophyton floccosum by derivative PB - 3 was equal to amphotercin B. [Conclusions] Some derivatives of pseudolaric acid B have stronger inhibitory effects on fungi than pseudolaric acid B and present a potential exploitation value.