NGF及其受体TrkA在胰腺癌嗜神经侵袭及疼痛发生的作用

目的 分析神经生长因子(NGF)及其受体酪氨酸激酶(TrkA)在胰腺癌嗜神经侵袭及疼痛发生的作用.方法 选择胰腺癌病例28例,根据术前患者的疼痛情况分为疼痛组(A组,14例)和无疼痛组(B组,14例).用Northern blot和免疫组化半定最检测并比较两组胰腺癌组织NGF及TrkA表达强度,并与神经侵袭程度评分和疼痛评分进行相关分析.结果 A组的神经侵犯评分明显高于B组,差异有统计学意义(P＜0.05).胰腺癌伴有外周神经侵犯者比无外周神经侵犯者的NGF和TrkA mRNA表达水平高,差异有统计学意义(P＜0.05),肿瘤伴有疼痛者比不伴有疼痛者的NGF和TrkA mRNA表达水平高,差异有统计学意义(这P＜0.05).免疫组化结果与上相似.外周神经侵犯的程度与NGF mRNA和TrkA mRNA表达水平呈正相关(r=0.622,P＜0.05;r=681.P＜0.05),疼痛程度与NGF mRNA和TrkA mRNA表达水平亦呈正相关(r=0.624,P＜0.05:r=0.632,P＜0.05).外周神经侵犯的程度与NGF和TrkA免疫组化评分呈正相关(r=602,P＜0.05;r=0.551,P＜0.05),疼痛程度与NGF和TrkA免疫组化评分亦呈正相关(r=603,P＜0.05;r=0.612,P＜0.05).结论 NGF及其受体TrkA在胰腺癌患者的疼痛发生和胰腺癌的嗜神经侵犯中起着重要作用.

Role of nerve growth factor/TrkA in perineural invasion and pain in pancreatic cancer

Objective To investigate the expressions of nerve growth factor(NGF)/TrkA in pancreatic cancer and evaluate their association with perineural invasion and pain in patients with pancreatic cancer. Methods Twenty-eight patients with pancreatic cancer were divided into group A wim pain(n=14)and group B without pain(n=14),and in all the patients the expressions of NGF/TrkA in the pancreatic CanCel tissue were detected by Northern blot analysis and immunohistochemistry.The molecular findings were analyzed in relation to the perineural invasion scores and pain scores.Results Perineural invasion scores in group A were significantly higher than that in group B (P＜0.05).Northern blot analysis revealed significantly higher NGF/TrkA levels and NGF/TrkA immunoreactivity in group A than in group B (P＜0.05).Immunohistochemistry showed that NGF was strongly expressedin the cytoplasm of the pancreatic cancer cells but TrkA Was localized in the perineurinm of the pancreatic Berve.NGF and TrkA mRNA expressions in the tumors were found to positively correlate to perineural invasion of the tumors(r=622,P＜0.05,and r=0.681,P＜0.05,respectively)and the pain scores(r=0.624,P＜0.05;r=0.632,P＜0.05).The immunohistochemical scores of NGF and TrkA expressions in the tumor were also positively correlated to perineural invasion of the tunlor(r=0.602,P＜0.05;r=0.551,P＜0.05)and the pain scores(r=q).603,P＜0.01;r=0.612,P＜0.01).Conclusions The NGF/TrkA system might play important roles in nerve infiltration by the tumor cells and pain sensation in pancreatic cancer patients.