Microchromatofocusing of hemoglobins. Increased hemoglobin A2 percentage in sickle cell trait

Observations on the suitability of frozen and lyophilized reference sera stored at ?20°C are presented. These sera, intended for use as controls for both cholesterol and triglyceride determinations, covered ranges for cholesterols from 4 to 8 mmol/1 and for triglyceride from 0.7 to 2.4 mmol/1. We observed no change in cholesterol or triglyceride concentration in either the frozen or lyophilized forms of serum during almost 5 years of storage. The suitability of these materials for cholesterol and triglyceride determinations is demonstrated by representative methods. Our results show that lyophilized reference sera can be shipped unprotected by dry ice without any effect on cholesterol or triglyceride concentration. Although the total cholesterol concentration was unchanged with storage, changes did occur in the lipoproteins carrying these lipid constituents.