| HPLC法测定湿热痹颗粒（减糖型）中盐酸小檗碱的含量 |
| |
| 引用本文： | 罗晶,滕怀凤,施法. HPLC法测定湿热痹颗粒（减糖型）中盐酸小檗碱的含量[J]. 中国医药导报, 2010, 7(14): 44-45 |
| |
| 作者姓名： | 罗晶 滕怀凤 施法 |
| |
| 作者单位： | 1. 抚顺市药品检验所,辽宁抚顺,113006
2. 辽宁省食品药品检验所,辽宁沈阳,110023 |
| |
| 摘 要： | 目的：建立高效液相法测定湿热痹颗粒（减糖型）中盐酸小檗碱含量的方法。方法：采用Diamonsil-C18（4.6mm×250mm,5μm）色谱柱;流动相：乙腈-1%磷酸溶液（48∶52）（每100ml中加入十二烷基磺酸钠0.1g）;检测波长：265nm;流速：1.0ml/min。结果：盐酸小檗碱在0.01028～0.41120μg范围内与峰面积呈良好的线性关系（r=0.9998）,平均回收率为98.30%,RSD=0.44%。结论：本方法简便、准确、重现性好,可用于湿热痹颗粒中盐酸小檗碱的质量控制。
|
| 关 键 词： | 湿热痹颗粒 盐酸小檗碱 高效液相色谱法 |
Content determination Of Berberine Hydrochloride in Shirebi Granules by HPLC |
| |
| LUO Jing,TENG Huaifeng,SHI Fa. Content determination Of Berberine Hydrochloride in Shirebi Granules by HPLC[J]. China Medical Herald, 2010, 7(14): 44-45 |
| |
| Authors: | LUO Jing TENG Huaifeng SHI Fa |
| |
| Affiliation: | 1.Institute for Drug Control of Fushun City,Fushun 113006,China;2.Institute for food and drug Control of Liaoning Province,Shenyang 110023,China) |
| |
| Abstract: | Objective：To establish a method for the content determination of berberine hydrochloride in Shirebi Granules by HPLC.Methods：The Diamonsil-C18（4.6 mm×250 mm,5 μm）column was used.The mobile phases consisted of acetonitrile and 1%-phosphoric acid（48：52）（to each of 100 ml add 0.1 g of sodium dodecylsulfonate）,the flow rate was 1.0 ml/min and the detection wavelength was at 265 nm.Results：The linear ranges of berberine hydrochloride was at 0.010 28-0.411 20 μg（r=0.999 8）,the average recovery was 98.30% with a RSD of 0.44%.Conclusion：The method is fast,reliable,accurate and can be used in the quality control of berberine hydrochloride in Shirebi Granules. |
| |
| Keywords: | HPLC Berberine hydrochloride Shirebi Granules Content determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
