黄连解毒汤对急性痔疮模型大鼠肛周病理组织形态、免疫功能以及炎症因子的影响

目的探讨黄连解毒汤对急性痔疮模型大鼠肛周病理组织形态、免疫功能以及炎症因子的影响。方法50只大鼠随机分为空白对照组、模型组、阳性组及黄连解毒高、低剂量组,每组10只。利用99.0%醋酸溶液制备急性痔疮大鼠模型,造模成功后,阳性组给予美沙拉嗪,黄连解毒组给予相应剂量黄连解毒汤灌胃。7 d后比较各组大鼠的肛周组织病理形态学变化及病理评分、免疫功能及细胞炎症因子。结果模型组大鼠的肛周组织呈暗红色,光泽度差,充血肿胀明显,肛周组织病变程度明显高于空白对照组,提示造模成功。治疗后,阳性组和黄连解毒组大鼠的组织光泽度得到明显改善,颜色变浅,充血肿胀程度和病变程度明显减轻,分泌物减少,其症状明显轻于模型组(P<0.05);模型组大鼠的溃疡面积和局部症状评分、TNF-α、IL-1β和IL-6水平以及CD8+明显高于空白对照组,CD3^+、CD4^+和CD4^+/CD8^+明显低于空白对照组(P<0.05)。阳性组和黄连解毒组的溃疡面积和局部症状评分、TNF-α、IL-1β和IL-6水平、CD8+明显低于模型组,CD3^+、CD4^+和CD4^+/CD8^+明显高于模型组(P<0.05)。阳性组与黄连解毒高剂量组的炎症因子和免疫功能比较无显著差异(P>0.05)。结论黄连解毒汤能有效地改善急性痔疮模型大鼠的病理组织形态,降低溃疡面积和局部症状评分,提高免疫功能,减少细胞炎症因子反应。

Effect of Huanglian Jiedu Decoction on Pathological Histomorphology,Immune Function and Inflammatory Factors in Rats with Acute Hemorrhoids

Objective:To investigate the effects of Huanglian Jiedu Decoction on the histopathological morphology,immune function and inflammatory factors in rats with acute hemorrhoids.Methods:50 rats were randomly divided into the control group,the model group,the positive group and the experimental groups(high dose and low dose),10 rats in each.Rat model of acute acne was made.After successful modeling,the positive group was given Mesalazine while the experimental groups were given Huanglian Jiedu Decoction.After 7 days,the pathological changes and pathological scores,immune function and cytokines of perianal tissues of rats in each group were compared.Results:The perianal tissues of the model group were dark red;the gloss was poor;the congestion and swelling were obvious,and the degree of perianal tissue was significantly higher than that of the control group.After treatment,the perianal tissue gloss of the positive and experimental groups had significant improvement.The color became lighter;the degree of hyperemia,swelling and pathological changes decreased;the secretion decreased,and the symptoms were better than those of the model group(P<0.05).The ulcer area and local symptom scores,TNF-α,IL-1β and IL-6 levels and CD8^+ in the model group were significantly higher than those in the control group,CD3^+,CD4^+and CD4^+/CD8^+ were significantly lower than the control group(P<0.05).After treatment,the ulcer area and local symptom scores,TNF-α,IL-1β and IL-6 levels and CD8+in the positive and experimental groups were significantly lower than those in the model group,CD3^+,CD4^+ and CD4^+/CD8^+ were significantly higher than the model group(P<0.05).There was no significant difference in inflammatory factors and immune function between the positive group and the high dose group(P>0.05).Conclusion:Huanglian Jiedu Decoction can effectively improve the pathological histomorphology of rats with acute hemorrhoids,reduce ulcer area and local symptom score,improve immune function and reduce cellular inflammatory factor response.