益气化痰活血方对AIT小鼠甲状腺Ets-1、IL-17基因表达的影响

目的:研究益气化痰活血方对自身免疫性甲状腺炎(AIT)小鼠甲状腺Ets-1、IL-17基因表达的影响,从分子水平说明其影响AIT的部分机制。方法:60只8周龄NOD.H-2^h4小鼠,随机分为正常组(NG),模型组(MG),西药组(SeG),益气化痰活血方高、中、低剂量组(YG-1、YG-2、YG-3),每组10只,正常组饲以蒸馏水,其余各组饲以含高碘化钠水(浓度0.05%)。8周后分别以蒸馏水,阳性对照药硒酵母混悬液,益气化痰活血方高、中、低剂量的水煎液给各组小鼠灌胃。8周后取小鼠甲状腺,RT-PCR法检测E26transformation-specific-1(Ets-1)、白细胞介素-17(IL-17) mRNA表达。结果:①与模型组比较,益气化痰活血方组均能使Ets-1 mRNA的表达水平上调(P<0.01);②与模型组比较,益气化痰活血方组均能使IL-17 mRNA的表达水平下调(P<0.01)。结论:益气化痰活血方能上调Ets-1基因表达水平,进而使IL-17炎症水平下降,这可能是其改善AIT小鼠免疫失常的机制之一。

Effect of Yiqi Huatan Huoxue Formula on Gene Expressions of Ets-1 and IL-17 in Thyroids of AIT Mice

Objective:To study the effect of Yiqi Huatan Huoxue Formula on the gene expressions of Ets-1 and IL-17 in thyroids of autoimmune thyroiditis(AIT)mice,thus to partially explain the potential molecular mechanism of the formula on AIT.Methods:A total of 60 NOD.H-2h4 mice(8-week-old)were randomly divided into the normal group(NG),the model group(MG),the western medication group(Selenious yeast,SeG),Yiqi Huatan Huoxue Formula of high-dose,medium-dose and low-dose(YG-1,YG-2 and YG-3),with 10 mice in each group.The mice in NG were fed with distilled water,and the others were supplied with 0.05%NaI for 8 weeks to induce AIT.After 8 weeks of decoction or sterile water administration,all mice were sacrificed and the thyroids were collected.Then the mRNA expressions of Ets-1 and IL-17 were detected by RT-PCR respectively.Results:Compared to the MG,the expression of Ets-1 mRNA was up-regulated(P<0.01),and the expression of IL-17 mRNA was down-regulated in Yiqi Huatan Huoxue Formula group(P<0.01).Conclusion:The probable mechanism of Yiqi Huatan Huoxue Formula on AIT mice with immune injury may be related to up-regulating Ets-1 mRNA expression and down-regulating IL-17 mRNA expression.