口腔粘膜癌前病变和鳞癌组织细胞增殖和凋亡的原位观察

目的研究自口腔正常粘膜上皮向异常增生和鳞癌组织演变过程中细胞增殖和凋亡的变化,阐明口腔癌发病机制.方法采用免疫组织化学S-P法和原位末端标记法检测10例正常口腔粘膜上皮、48例异常增生上皮和42例鳞癌组织中增殖细胞数和凋亡数.结果异常增生上皮细胞增殖和凋亡数均明显高于正常组(P＜0.05),但随着增生程度的加重,伴随着细胞增殖能力逐级增强,细胞凋亡数无明显提高(P＞0.05).鳞癌组织中,随着组织学分级的增加,增殖数明显增多,凋亡数逐级减少.结论癌前病变中,细胞凋亡速度增加跟不上增殖速度,导致向鳞癌转变.癌细胞增殖不断加强,凋亡不断下降,两者共同作用的结果导致鳞癌的发展.

Cell apoptosis and proliferation in oral cancer and precancerous lesions

Objective To study the alteration of cell proliferation and cell apoptosis during the development of oral squamous cell carcinoma (SCC).Methods The number of cell proliferation (Ki67) and apoptosis (AI) was observed in 10 normal oral epithelial,48 epithelial dysplasia and 42 SCC by immunohistochemical and TUNEL evaluation.Results In dysplastic epithelia,the number of Ki67 and AI was more than that of normal epithelia,and with the dysplasis increasing,the Ki67 number increased significantly,while the AI had no significant elevation.In SCC,with the differentiation decreasing,the Ki67 number was increased,the AI was decreased.Conclusion The increasing rate of cell apoptosis can't keep up with the rate of cell proliferation,which induces the change towards oral SCC.The proliferation of cancer cell become more and more intensive,while the apoptosis are lastingly down-regulated.Both factors will result in the development of SCC.