生存蛋白在胃癌中的表达及与p53、c-erbB2表达的关系

目的　研究人胃癌组织中凋亡抑制蛋白 (IAP)家族的生存蛋白 (survivin)的表达与临床病理及与 p5 3和c erbB2表达的关系。方法　采用免疫组化的方法检测 5 6例胃癌患者肿瘤组织及 2 0例慢性胃炎患者胃粘膜中survivin和 p5 3、c erbB2的表达。结果　 2 0例慢性胃炎患者胃粘膜中survivin的表达阳性 1例 ,阳性率为 5 % ;5 6例胃癌组织中survivin阳性 2 7例 ,阳性率 4 8.2 % ;5 6例胃癌患者c erbB2阳性率 35 .7% (2 0 / 5 6 ) ,p5 3阳性率39.3% (2 2 / 5 6 )。survivin表达与患者年龄、肿瘤浸润深度、淋巴结转移、临床分级等无明显相关性 ,而与肿瘤Lauren组织分型相关。survivin阳性率在肠型胃癌 (2 0 / 32 ,6 2 .5 % )中显著高于弥漫型 (7/ 2 4 ,2 9.2 % ,P <0 .0 5 )。survivin在c erbB2阳性和阴性患者中阳性率分别为 80 % (16 / 2 0 )和 30 .6 % (11/ 36 ) ,两者间有显著差异 (P <0 .0 1) ;survivin在 p5 3阳性和阴性患者中阳性率分别为 6 8.2 % (15 / 2 2 )和 35 .3% (12 / 34) ,两者比较也有显著差异 (P <0 .0 5 )。结论　胃癌患者survivin高表达导致的凋亡抑制在胃癌的发生发展中起了重要作用 ,survivin表达与胃癌中 p5 3和c erbB2异常表达相关。

Expression of Antiapoptosis Gene,Survivin,Correlated with the Expression of p53 and c-erbB2 in Gastric Carcinoma

Purpose To study the expression of survivin,an inhibitor of apoptosis protein,in gastric carcinomas and its relationship with the expression of p53,c erbB2. Methods Using immunohistochemical staining method,we examined the expression of survivin,p53 and c erbB2 genes in 20 cases of chronic gastritis and 56 cases of gastric carcinomas. Results Survivin expressed in 27 of 56(48.2%) cases of gastric carcinoma tissues and 1 of 20(5%) cases of chronic gastritis.Over expression of survivin had no relation with age,tumor depth,tumor size,and disease stage,but was significantly related to histological type.The expression of survivin significantly segregated with intestinal type cases as compared with diffuse type cases.Survivin positive cases were significantly associated with p53 expression(15/22,68.2% versus 12/34,35.3%, P <0.05) and c erbB2 expression(16/20,80% versus 11/36,30.6%, P <0.01). Conclusions These data indicated that survivin play an important role in the onset of gastric cacinoma and suggested potential correlation between survivin and c erbB2,and between survivin and p53 as well.