| 中药双黄补对人牙龈成纤维细胞损伤的保护作用 |
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| 引用本文： | 许彦枝,吴景景,刘健,陈彦平. 中药双黄补对人牙龈成纤维细胞损伤的保护作用[J]. 中国医院药学杂志, 2011, 0(16): 1325-1329 |
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| 作者姓名： | 许彦枝 吴景景 刘健 陈彦平 |
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| 作者单位： | 河北医科大学第四医院; |
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| 基金项目： | 国家自然科学基金面上项目(编号:30873289) |
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| 摘 要： | 目的:观察中药双黄补对人牙龈成纤维细胞由机械划痕损伤、内毒素脂多糖(LPS)损伤所致的保护作用.方法:采用组织块法体外培养人牙龈成纤维细胞.水提醇沉法制备双黄补提取液.采用四甲基偶氮唑蓝法测定培养细胞增殖活性的变化.建立体外细胞划痕创伤模型,加入双黄补培养24 h观察细胞迁移.绘制生长曲线观察双黄补对LPS介导的成纤维...
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| 关 键 词： | 双黄补 牙龈成纤维细胞 增殖 损伤 保护 |
Protective effect of Chinese medicine Shuanghuangbu on human gingival fibroblasts injury |
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| XU Yan-zhi,WU Jing-jing,LIU Jian,CHEN Yan-ping. Protective effect of Chinese medicine Shuanghuangbu on human gingival fibroblasts injury[J]. Chinese Journal of Hospital Pharmacy, 2011, 0(16): 1325-1329 |
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| Authors: | XU Yan-zhi WU Jing-jing LIU Jian CHEN Yan-ping |
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| Affiliation: | XU Yan-zhi,WU Jing-jing,LIU Jian,CHEN Yan-ping(The Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China) |
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| Abstract: | OBJECTIVE To observe the protective effect of Chinese medicine Shuanghuangbu on human gingival fibroblasts(HGFs) injury induced by mechanical and LPS injury.METHODS HGFs were cultured in vitro by using the method of tissue piece.Shuanghuangbu extract was prepared with water extraction and alcohol precipitation method.Proliferation activity of HGFs was determined by MTT.The in vitro cell scratch wound model was set up to observe the effect of Shuanghuangbu on the migration of HGFs after 24 hours of culture.B... |
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| Keywords: | Shuanghuangbu human gingival fibroblasts proliferation injury protection |
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