| HPLC法测定不同产地莕菜中齐墩果酸和熊果酸的含量 |
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| 引用本文： | 蔡,颖.HPLC法测定不同产地莕菜中齐墩果酸和熊果酸的含量[J].药学与临床研究,2012,20(1):94-96. |
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| 作者姓名： | 蔡 颖 |
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| 作者单位： | 南京市白下建中中医院,南京,210004 |
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| 摘 要： | 目的:建立HPLC法测定莕菜中齐墩果酸和熊果酸的含量的方法,并对不同产地的药材进行测定.方法:采用Shim-pack CLC-ODS(M)(4.6 mm×250 mm,5μm)色谱柱；流动相为甲醇-水-磷酸(86∶14∶0.02)；检测波长为210 nm;流速为1.0 mL· min-1；柱温为20℃.结果:齐墩果酸和熊果酸分别在1.02～20.40 μg· mL-1、0.53～10.60 μg· mL-1范围内峰面积与浓度呈良好的线性关系.结论:该方法简便、准确,可以用于善菜药材的质量控制.
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| 关 键 词： | HPLC法 莕菜 齐墩果酸 熊果酸 |
| 收稿时间： | 2011/10/19 0:00:00 |
| 修稿时间： | 2011/12/26 0:00:00 |
Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Herba Nymphoides peltatdum from Different Habitats by HPLC |
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| CAI Ying.Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Herba Nymphoides peltatdum from Different Habitats by HPLC[J].Pharmacertical and Clinical Research,2012,20(1):94-96. |
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| Authors: | CAI Ying |
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| Institution: | CAI YingJianzhong Traditional Chinese Medicine Hospital of Baixia Area,Nanjing 210004 |
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| Abstract: | Objective: To establish the HPLC method for simultaneously determination of oleanolic acid and ursolic acid in Herba Nymphoides peltatdum(Gmel.) O.Kuntze from different habitats.Methods: A shim-pack CLC-ODS(M) column(4.6 mm×250 mm,5 μm) was used,the mobile phase was as methanol-water-phosphate(86∶14∶0.02),the detection wavelength was at 210nm,the flow rate was at 1.0 mL·min-1,the column temperature was at 20℃.Results: The linear rang of oleanolic acid was 1.02~20.4 μg·mL-1(r=0.9997),the linear rang of ursolic acid was 0.53～10.60 μg·mL-1(r=0.9998).Conclusion: The method is simple,rapid and accurate.It can be used for quality control of Herba Nymphoides peltatdum(Gmel.) O.Kuntze. |
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| Keywords: | HPLC Nymphoides peltatdum Oleanolic acid Ursolic acid |
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