RP-HPLC法测定痹痛宁胶囊中士的宁和马钱子碱的含量

采用反相高效液相色谱法测定痹痛宁胶囊中士的宁和马钱子碱的含量。色谱柱CAP-CELL C18(4.6 mm×150 mm,5μm);流动相:水-乙腈(80∶20)(含三乙胺0.01 mol·L-1,用磷酸调pH至2.6±0.1);检测波长260 nm;流速1.0 mL·min-1;柱温25℃。测得士的宁和马钱子碱的浓度分别为0.2097～3.1455μg(r=0.9996)和0.0952～1.4280μg(r=0.9994),平均回收率(n=9)分别为99.2%、99.3%。

Determination of Strychnine and Brucine in Bitongning Capsules by RP-HPLC with Ultraviolet Detection

An HPLC method has been established for the determination of strychnine and brucine in Bitongning Capsule.A Capcell C18 column(150 mm×4.6 mm,5 μm) was used.The mobile phase consisted of a methyl cyanides-0.01 mol·L-1 phosphate buffer solution(20∶80,v/v;pH 2.6±0.1) and flowed at 1.0 mL·min-1.The column temperature was 25℃.The detection wavelength was at 260 nm and the injection volume was 20 μL.The method had good linear relationship in the range of 0.2097～3.1455 μg(r=0.9996) for strychnine and 0.0952～1.4280 μg(r=0.9994) for brucine,respectively.The results of the average recovery were 99.2% for strychnine and 99.3% for brucine.