同时进行原位杂交及染色体分带的基因定位新方法

作者报道一种同时进行染色休原位杂交及染色休分带的基因定位新方法。它具有简单、快速、准确以及结果稳定、易重复等优点。细胞经常规培养制成染色体玻片后，进行染色体原位杂交。杂交信号经抗体检测放大后，染色体依次用色霉素Ａ３以及甲绿处理进行荧光反带分带（Ｒ带）。玻片在荧光显微镜下观察，只要调换滤片即可分别观察杂交点及染色体Ｒ带。两者互不干扰，结果清晰、直观，杂交点及Ｒ带持久而稳定。此方法尤其适用于做基因定位，及检测有关病毒基因在受感染细胞染色体上的插入部位。

A NOVEL GENE MAPPING METHOD FOR SIMULTANEOUS IN-SITU HYBRIDIZATION AND CHROMOSOME BANDING

A new method for simultaneous in-situ hybridization and chromosome banding for gene mapping was reported.It was simple, rapid and stable. Cells were cultured according to the routine method.In-situ hybridization was made. After the detection of hybridization signals with antibodies,chromosome banding was performed with chromomycin A3 and methylgreen (R banding).Under the fluorescent microscope,hybridization signals and chromosome banding were observed by changing the filters.The two steps didn't interfere with each other and the results were very clear-cut.The method is specially suitable for gene mapping and the detection of viruses and their inserting sites on chromosomes of infected host cells.