芹菜素对NCI-H446细胞系球细胞自我更新和uPAR表达的影响

目的：研究芹菜素对人小细胞肺癌NCI-H446细胞系球细胞自我更新的影响及机制。方法：采用干细胞条件培养基超低黏附板悬浮培养法从体外培养NCI-H446细胞系得到球细胞。测定球形成率用以评价0(0.1% DMSO对照组)，5.0，10.0，20.0 μmol/L芹菜素对NCI-H446细胞系球细胞自我更新的抑制作用；Western印迹分析球细胞尿激酶型纤维蛋白酶原激活物受体(urokinase-type plasminogen activator receptor，uPAR)的表达。结果：0，5.0，10.0，20.0 μmol/L芹菜素呈剂量依赖性抑制NCI-H446细胞系第2代球细胞的球形成率[球形成率分别为(18.2±1.9)%，(13.6±1.7)%，(10.6±1.6)，(6.9±1.3)%]，并呈浓度依赖性下调uPAR蛋白的表达(P<0.05)。结论：芹菜素抑制人小细胞肺癌NCI-H446细胞系球细胞自我更新作用，可能与下调uPAR表达相关。

Effects of apigenin on self-renewal and #br#uPAR expression in NCI-H446 cell line

Objective: To investigate the effect of apigenin on self-renewal for sphere-forming cells in human small cell lung cancer cell line NCI-H446 and the underlying mechanisms.Methods: Sphere-forming cells from NCI-H446 cell line were cultured in stem cell-conditioned culture medium with ultra-low attachment surface plates. The rate of sphere-forming cells in the second passage sphere-forming cells was used to evaluate the inhibitory effects of apigenin on the self-renewal for sphere-forming cells. The protein level of urokinase-type plasminogen activator receptor (uPAR) in spheroids was analyzed by Western blot.Results: Apigenin signifcantly inhibited the self-renewal of the second passage sphere-forming cells [0, 5.0, 10.0, 20.0 μmol/L apigenin: (18.2±1.9)%, (13.6±1.7)%, (10.6±1.6)%, (6.9±1.3)%, respectively] and down-regulated uPAR expression in a concentration-dependent manner (P<0.05).Conclusion: Apigenin inhibits the self-renewal capacity of sphere-forming cells in NCI-H446 cells, which may be associated with down-regulation of uPAR expression.