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| 信号转录激活子3基因shRNA慢病毒载体的构建与鉴定 | |
| 引用本文： | 阮坚丽,高源,管文贤,乔志明,钱伟峰. 信号转录激活子3基因shRNA慢病毒载体的构建与鉴定[J]. 南通医学院学报, 2010, 30(2): 83-85 |
| 作者姓名： | 阮坚丽 高源 管文贤 乔志明 钱伟峰 |
| 作者单位： | 南京医科大学附属苏州市立医院急诊科,苏州,215002 |
| 基金项目： | 苏州市社会发展计划项目(SS08033) |
| 摘 要： | 目的：构建人信号转录激活子3（STAT3）基因shRNA慢病毒载体。方法：从GenBank STAT3 mRNA上寻找到3条有效靶序列,合成3对针对靶序列的siRNA oligo,通过筛选获得最佳靶序列后合成相应的shRNA模板,将合成好的两条互补的DNA单链退火形成双链DNA,经与BamH Ⅰ和Xho I酶切的pRNAT-U6.2/Lenti载体连接后产生shRNA慢病毒载体,酶切和测序鉴定。结果：酶切和测序鉴证实合成STAT3shRNA慢病毒载体寡核甘酸链插入正确。结论：成功构建人STAT3基因shRNA慢病毒载体。 |
| 关 键 词： | 信号转录激活子3 RNA干扰 慢病毒 |
Construction of STAT3 shRNA lentiviral vector |
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| RUAN Jianli,GAO Yuan,GUAN Wenxian,et al. Construction of STAT3 shRNA lentiviral vector[J]. ACTA Academiae Medicinae Nantong, 2010, 30(2): 83-85 | |
| Authors: | RUAN Jianli GAO Yuan GUAN Wenxian et al |
| Affiliation: | Suzhou Muniwpal Hospital Affiliated to Nanjing Medical University/a>;Suzhou 215002 |
| Abstract: | Objective:To construct a reco mbinant lentiviral vector of RNA interference of STAT3 gene.Methods:Three different siRNAs of STAT3 were designed and synthesized.After being screened,the most effective siRNA was found.According to this sequence,the short hairpin DNA of STAT3 was constructed.The shRNA duplex was ligated into the recombinant vector pRNAT-U6.2/Lenti.The recombine vector was confirmed by enzyme cutting and DNA sequencing.Results:The restriction map and DNA sequencing demonstrated that the recombi... |
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| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |