HBV基因型在HBV感染性肝癌及慢性HBV感染自然史不同阶段的分布

目的探讨乙型肝炎病毒(hepatitis B virus,HBV)基因型在HBV相关性肝细胞癌(hepatocellular carcinoma,HCC)及慢性HBV感染自然史不同阶段的分布。方法选择广西桂北地区46例HBV感染相关性HCC患者,慢性HBV感染自然史不同阶段者共90例,通过实时荧光定量PCR鉴定HBV基因型。临床实验室检查结果和病理资料摘抄自患者病案。结果本地区慢性HBV感染者中基因型B占77.8%,基因型C占18.9%,非B非C占3.3%;HCC中基因型B占78.3%,基因型C占21.7%,慢性HBV感染者和HCC之间的基因型分布差异无统计学意义(P>0.05)。慢性HBV感染不同阶段者之间的基因型分布差异无统计学意义(P>0.05)。慢性HBV感染者中基因型B、C比较,临床资料差异无统计学意义(P>0.05),HCC中基因型B、C间HBV-DNA比较差异有统计学意义(P<0.01)。慢性HBV感染不同阶段组和HCC组比较,ALT、AST、TBIL、ALB、年龄,性别差异均有统计学意义(P<0.05)。结论广西桂北地区的慢性HBV感染者和HBV相关性HCC以基因型B为主,基因型在HBV感染自然史不同阶段和HCC对比分布无差别。基因型C的HBV-DNA水平更高。

Distribution of HBV genotypes in HBV infection-associated hepatocellular carcinoma and in different phases of natural chronic HBV infection history

Objective To study the distribution of HBV genotypes in HBV infection-associated hepatocellular carcinoma(HCC) and in different phases of natural chronic HBV infection history.Methods HBV genotypes in 46 patients with HBV infection-associated HCC and 90 patients with a natural chronic HBV infection history at different phases in the Northern Guangxi Zhuang Autonomous Region were identified by real-time fluorescence quantitative PCR.Laboratory findings and pathological data were obtained from medical records of the patients.Results Genotypes B,C,and non-A and B accounted for 77.8%,18.9%,and 3.3%,respectively,in patients with chronic HBV infection.Genotypes B and C accounted for 78.3% and 21.7% in HCC patients.No significant difference was observed in distribution of HBV genotypes among the patients with HBV infection,HCC,and chronic HBV infection at different phases(P>0.05).A significant difference was found in HBV-DNA between genotypes B and C(P<0.01).The ALT,AST,TBIL,ALB,and age levels were significantly higher in HCC patients than in those with chronic HBV infection at different phases(P<0.05).Conclusion Genotype B is dominant in patients with chronic HBV infection and HBV infection-associated HCC in the Northern Guangxi Zhuang Autonomous Region with no difference in distribution of HBV genotypes in patients with HCC and a natural chronic HBV infection history at different phases.The HBV-DNA level is higher in genotype C than in other HBV genotypes.