灵芝益甘粉对D-氨基半乳糖诱导小鼠肝损伤的保护作用研究

目的:观察灵芝益甘粉保肝作用,并探讨其作用机制。方法:实验分为:正常对照组、模型对照组、阳性对照组、灵芝益甘粉高、中、低剂量组。造模24h后处死动物并取材,测定:①肝、脾、胸腺与体重之比;②血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;③肝组织丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)含量;④小鼠肝组织病理变化。结果:灵芝益甘粉低、中、高剂量给药组对模型小鼠升高的ALT和AST活性降低。与模型组比较,灵芝益甘粉可以明显降低小鼠肝匀浆MDA的水平,升高GSH-PX活性。模型组小鼠肝细胞气球样变性、坏死程度高于对照组,灵芝益甘粉能明显改善肝细胞坏死病变程度。结论:灵芝益甘粉对D-氨基半乳糖诱导的肝损伤具有一定的保护作用,其保护作用可能与其抑制脂质过氧化损伤有关。

The Protective Effect of Ganoderma Liver-protective Powder on D-galactose Induced Liver Injury in Mice

Objective： To observe the effect of Ganoderma liver-protective powder （GLPP） on liver protection and explore the mechanism. Methods： Experimental animals divided into four group e. g. control group, model group, positive control group, GLPP high,medium and low-dose groups. After 24 hours modeling, we kill the mice and take samples： （Dcalculated the ratio of liver, spleen, thymus with body weight;Qdetected the activity of serum alanine aminotransferase（ALT）, aspartate aminotrans- ferase （AST） ;Qdeteetedthe content of liver maleic dialdehyde （MDA） and glutathione peroxidase（GSH-PX） ;@observed histo- pathology of liver were. Results： The low-dose, medium-dose and high-dose of GLPP decreased the elevating activity of ALT and AST. The low-dose, medium-dose and high-dose of GLPP could obviously decrease the content of MDA of liver homogenate, The activity of GSH-PX in liver homogenate increased. Histopathological changes in liver tissue show that, there are balloon-lik~e de- generation in liver cells and cellular necrosis in model and the degree higher than control. GLPP could obviously improve the de- gree of hepatic necrosis. Conclusion： GLPP showed significant protective action on acute liver injury induced by D-GalN in mice, and protective effect may be related to the inhibition of lipid peroxidation damage.