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| SBR基因原核表达质粒的构建 | |
| 引用本文： | 张苗,陈洪雷,姜广水,杨丕山,卞继峰.SBR基因原核表达质粒的构建[J].泰山医学院学报,2008,29(2):90-92. |
| 作者姓名： | 张苗 陈洪雷 姜广水 杨丕山 卞继峰 |
| 作者单位： | 1. 解放军第88医院,山东,泰安,271000 2. 山东大学口腔医学院,山东,济南,250012 3. 山东大学西校区分子生物学教研室,山东,济南,250012 |
| 摘 要： | 目的构建可用于大肠杆菌表达系统的含变形链球菌唾液结合区段(SBR)基因的表达载体pcMVT7-SBR。方法用定向克隆方法将SBR基因插入表达载体pcMVT7,构建重组原核表达质粒pcMVT7-SBR。结果经酶切鉴定及DNA序列测定,重组质粒pcMVT7-SBR序列及阅读框架正确。结论SBR表达载体构建成功,为防龋疫苗的动物实验研究奠定基础。 |
| 关 键 词： | 唾液结合区段 构建 防龋疫苗 |
| 文章编号： | 1004-7115(2008)02-0090-03 |
| 修稿时间： | 2007年10月8日 |
Construction of prokaryotic expression vector of SBR gene |
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| ZHANG Miao,CHEN Hong-lei,JIANG Guang-shui,YANG Pi-shan,BIAN Ji-feng.Construction of prokaryotic expression vector of SBR gene[J].Journal of Taishan Medical College,2008,29(2):90-92. | |
| Authors: | ZHANG Miao CHEN Hong-lei JIANG Guang-shui YANG Pi-shan BIAN Ji-feng |
| Institution: | ZHANG Miao; CHEN Hong-lei; JIANG Guang-shui; YANG Pi-shan; BIAN Ji-feng(The 88th Hospital of PLA; Taian 271000; China); |
| Abstract: | Objective: To obtain the prokaryotic expression vector containing the saliva binding region(SBR) gene of Streptococcus mutans.Methods: By directional cloning method,SBR gene fragment was cloned into the expression vector pcMVT7 and recombinant prokaryotic expression plasmid pcMVT7-SBR with right reading frame was successfully constructed.Results: Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA.The results were in correspondence with the initial design... |
| Keywords: | SBR construction anti-caries vaccine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |