运动对快速老化小鼠海马突触素表达的影响

目的研究运动对快速老化小鼠（SAMP8）海马突触素表达的影响,探讨运动改善阿尔茨海默病（AD）学习记忆功能的机制。方法 3月龄SAMP8小鼠40只随机分为运动组（采用跑笼运动训练）和对照组,2个月后HE染色观察2组海马神经元形态改变;免疫组化技术检测2组海马突触素表达。结果 5月龄SAMP8小鼠对照组海马部分神经元细胞变性、死亡,核浓缩,空泡变性;运动组偶有神经元细胞变性、死亡,大部分细胞形态正常。海马突触素表达运动组较对照组显著增高（P〈0.05）。结论运动可以延缓SAMP8小鼠海马神经元变性,提高海马突触素表达,这可能是运动改善AD学习记忆功能的重要机制之一。

Effects of movement on hippocampus synaptophysin of senescence-accelerated mouse

Objective To investigate the effect of movement on hippocampus synaptophysin of SAMP8(senescence-accelerated mouse/prone) mouse.Methods Forty 3-mouth-old SAMP8 mice were randomly divided into movement group and control group.The morphological changes of hippocampus neuron were observed under microscope after HE staining.The expression of synaptophysin immunoreactive cell in hippocampus was detected by immumohistochemistry method two months later.Results HE staining results showed that in control group there was neuron degeneration and death,chromatin condensation and vacuolar degeneration in hippocampus of 5-mouth-old SAMP8 mice.In movement group,there was few neuron degeneration and death,and most cells’ morphous were normal.Through immumohistochemistry staining,the expression of synaptophysin immunoreactive cell in hippocampus of 5-mouth-old SAMP8 mice in movement group(0.227±0.019) were significantly higher than that(0.156±0.008) in control group(P<0.05).Conclusion Movement can delay neuron degeneration and up-regulate synaptophysin expression levels in hippocampus of SAMP8 mice,which may be an important mechanism of improving learning and memory ability through movement in patients with Alzheimer’s disease.