Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells |
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| Affiliation: | 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China;2. College of Life Technology, Shihezi University, Shihezi 832003, Xinjiang, China;3. College of Medicine, Shihezi University, Shihezi 832003, Xinjiang, China;1. Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain;2. Departamento de Anatomía y Producción Animal, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain;3. Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain;1. Laboratorio de Genética Molecular “Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela;2. Departamento de Anatomía Patológica, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela;3. Centro de Biotecnología, Instituto de Estudios Avanzados, Caracas, Venezuela;1. Taian City Central Hospital, Taian, Shandong, China;2. Department of General Surgery, The Third People''s Hospital of Chengdu, The Second Affiliated Hospital of Chengdu, Chongqing Medical University, Chengdu, Sichuan, China;3. School of Preclinical Medicine, Chengdu Medical College, Chengdu, Sichuan, China;4. School of Medicine, Shihezi University, Shihezi, Xinjiang, China;1. Nucleo de Medicina Tropical, Universidade Federal do Para, Belem-Pa, Brazil;2. Centro de Ciências Biologicas e da Saude, Universidade do Estado do Para, Belem-Pa, Brazil;1. Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA;2. Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA;3. Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA;4. Molecular and Cell Biology Program, College of Science, Oregon State University, Corvallis, OR 97331, USA |
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| Abstract: | ![]()
Macroautophagy (autophagy) is an evolutionarily conserved control process that maintains cellular homeostasis in eukaryotic cells. Autophagy principally serves an adaptive role to degrade dysfunctional proteins and to clean damaged organelles in response to pathogenic, viral, or microbial infection, nutrient deprivation and endoplasmic reticulum (ER) stress. In previous study, we showed bovine viral diarrhea virus (BVDV) NADL infection induced autophagy and significantly elevated the expression levels of autophagy-related genes, Beclin1 and ATG14, at 12 h post-infection in MDBK cells. However, the specific mechanisms involved in controlling autophagic activity remain unclear. Here, we investigate the effects of BVDV NADL envelope glycoproteins overexpression on inducing autophagy. The results show that viral envelope glycoproteins Erns and E2 overexpression mediated by lentivirus increase the formation of autophagosome, the percentage of GFP-LC3 puncta-positive cells and the expression levels of Beclin1 and ATG14. Whereas E1 overexpression doesn't affect autophagic activity. Collectively, these findings suggest that the viral envelope glycoproteins Erns and E2 are involved in inducing autophagy, and provide a mechanistic insight into the regulation of autophagy in viral infected cells. |
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| Keywords: | Bovine viral diarrhea virus Autophagy Autophagy" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" macroautophagy 50% tissue culture infective dose GFP-LC3" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" green fluorescent-microtubule-associated protein light chain 3 fusion protein MOI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" multiplicity of infection BVDV" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" bovine viral diarrhea virus ATG" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" autophagy-related gene ER" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" Endoplasmic reticulum |
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