PAI-1基因多态性与2型糖尿病的相关性

目的分析2型糖尿病患者（T2DM）纤溶酶原激活物抑制物（PAI）-1基因型及分布频率，探讨PAI-1基因多态性与T2DM的关系。方法观察组为34例T2DM患者，对照组为39例健康体检正常者，采用等位基因特异性引物PCR扩增技术检测各组人群PAI-1基因多态性，两组人群血浆PAI-1含量检测采用美国USCNLIFE原装人PAI-1 ELISA试剂盒，酶标仪450nm波长下测定吸光度（OD值），两次平行测定取均值计算样品PAI．1水平。结果观察组与对照组相比PAI-1基因型频率及等位基因频率均有显著性差异（r=24．127，P〈0．001；X^2=7．312，P〈0．05）。观察组4G等位基因频率明显高于对照组，有显著性差异（X^2=6．280，P〈0．05）。观察组血浆PAI-1水平明显高于对照组（t=10．7，P〈0．01），观察组内比较4G／4G者血浆PAI-1含量明显高于4G／5G和5G／5G者，有显著性差异（F=21．02，P〈0．001）。结论PAI-1基因多态性与T2DM间存在一定的关联性，其中4C等位基因可能是T2DM发病的危险因子。

Relationship between the gene polymorphism of PAI-1 and type 2 diabetes mellitus

Objective To analyze the gene type and distribution frequency of plasminogen activator inhibitor type-1 （PAI-1） in patients with type 2 diabetes mellitus （T2DM）, and to explore the relationship between the gene polymorphism of PAI-1 and T2DM. Methods Thirty-four patients with T2DM （observed group） and 39 healthy controls （control group） were enrolled in the study. The gene polymorphism of PAI-1 in two groups was detected by specific polymerase chain reaction （PCR）, and plasma level of PAI-1 was measured by ELISA. Results There were significant differences in PAI-1 gene types frequency and allele frequency between the two groups （X^2 = 24. 127, P 〈 0. 001 ;X^2 = 7.312, P 〈 0.05 ）. The frequency of 4G allele frequency in the observed group was significantly higher than that in the control group （X^2 = 6. 280 ,P 〈 0.05 ）. The plasma level of PAl-1 in the observed group was significantly higher than that in the control group（ t = 10.7 ,P 〈 0.01 ）, and which in the patients with gene type of 4G/4G was significantly higher than that in the patients with gene type of 4（;/5 G 和 5G/5G（ F = 21.02 ,P 〈 0.001 ）. Conclusion There lies a recorrelation between the gene polymorphism and T2DM, the 4G allele of PAI-1 maybe a risk factor for the generation of T2DM.