大鼠舌下腺TFF2、TFF3在实验性胃溃疡愈合过程中表达的变化

目的探讨舌下腺三叶因子2(TFF2)和三叶因子3(TFF3)在大鼠实验性胃溃疡自愈期间表达的变化。方法雄性SD大鼠分为溃疡组(42只)和正常对照组(6只)。溃疡组在胃前壁近胃窦处将0.01ml冰醋酸注入黏膜下层制作实验性胃溃疡模型,对照组不作任何处理。分别以免疫组织化学S-P法和RT-PCR检测溃疡组和正常组舌下腺TFF2及TFF3基因和多肽表达情况。结果 TFF2及TFF3免疫反应阳性物质主要位于纹状管、闰管和肌上皮细胞,管腔内亦有阳性物质表达。TFF2的积分光密度在溃疡第1天时与正常组相比明显增高,第2天最低,第4、第6天逐渐升高(P<0.01),第10～23天均维持在较高水平(P<0.05);而TFF3的积分光密度在溃疡第1、第2天时接近正常组,第4、第6天逐渐增强并高于正常组(P<0.05),到第10天达高峰(P<0.01),至第23天仍高于正常组(P<0.05)。RT-PCR显示TFF2mRNA和TFF3mRNA的转录情况与相应多肽的表达趋势相似。结论舌下腺TFF2和TFF3基因在大鼠实验性胃溃疡自愈期间表达增高,可能参与胃溃疡愈合过程的调节。

Changes of TFF2 and TFF3 expression in sublingual gland during healing of experimental gastric ulcer in rats

Objective To investigate the changes of TFF2and TFF3expression in sublingual gland during spontaneous healing of experimental gastric ulcer in rats.Methods A total of 48male SD rats were divided into gastric ulcer group(n=42,ulcers were induced by injection of acetic acid to the submucosal of paries anterior gastricus)and normal group(n=6).Immunohistochemical and RT-PCR methods were used to examine the expression of TFF2and TFF3in the sublingual gland in gastric ulcer group and normal group.Results Immunohi...