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| L5前根切断大鼠模型中背根神经节P2X_3受体表达 | |
| 引用本文： | 周蓓,代娟丽,张德仁. L5前根切断大鼠模型中背根神经节P2X_3受体表达[J]. 儿科药学杂志, 2009, 15(3) |
| 作者姓名： | 周蓓 代娟丽 张德仁 |
| 作者单位： | 广东医学院附属深圳南山医院,广东深圳,518052 |
| 基金项目： | 深圳市科技计划项目(医疗卫生类) |
| 摘 要： | 目的:探讨L5前根切断(L5 VRT)大鼠神经病理性疼痛模型背根神经节(DRG)P2X3受体表达.方法:雄性SD大鼠30只,随机分为5组(n=6).A组(L5 VRT术后3 d)、B组(L5 VRT术后7 d)、C组(L5 VRT术后14 d)、D组(L5 VRT术后21 d)模型组和E组(假手术组),E组仅行半椎板切除术暴露左侧脊髓,分别在各自的时间点灌注取材L5背根神经节,用免疫荧光染色方法观察P2X3受体表达的变化.结果:(1)L5前根切断手术后,大鼠从次日开始即能产生双足明显的机械痛敏,与E组相比差异有统计学意义(P<0.05),这种痛敏情况持续至少4周;(2)免疫荧光染色发现B组、C组及D组模型组同侧L5 DRG的P2X3受体的表达明显增加(P<0.01),B组、C组及D组模型组对侧L5 DRG的P2X3受体的表达与E组相比变化不明显(P>0.05);A组模型组的双侧L5 DRG的P2X3受体的表达与E组相比无明显变化(P>0.05).结论:(1)L5前根切断疼痛模型能产生双足明显的机械痛敏;(2)该模型背根神经节P2X3受体的表达变化可能参与其机械痛敏的机制. |
| 关 键 词： | P2X3受体 神经病理性疼痛 L5前根切断 背根神经节 |
P2X_3 Receptor Expression in Dorsal Root Ganglion in Rats with Lumbar 5 Ventral Root Transection |
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| ZHOU Bei,DAI Juan-li,ZHANG De-ren. P2X_3 Receptor Expression in Dorsal Root Ganglion in Rats with Lumbar 5 Ventral Root Transection[J]. Journal of Pediatric Pharmacy, 2009, 15(3) | |
| Authors: | ZHOU Bei DAI Juan-li ZHANG De-ren |
| Affiliation: | ZHOU Bei,DAI Juan-li,ZHANG De-ren (Shenzhen Nanshan Hospital Affiliated to Guangdong Medical College,Guangdong Shenzhen 518052,China) |
| Abstract: | Objective:To observe the P2X3 receptor expression in lumbar 5 dorsal root ganglion(L5 DRG)with lumbar 5 ventral root transection (L5 VRT). Methods:Thirty male Sprague-Dawley rats were randomly divided into 5 groups,group A 3 days after L5 VRT,group B 7 days,group C 14 days,group D 21 days and group E of sham operation,whose left spine cords were exposed by hemilaminectomy. Rats in each group were respectively perfused with paraformaldehyde and materials were taken from L5 dosal root ganglions and the change... |
| Keywords: | P2X3 receptor Neuropathic pain Lumbar 5 ventral root transection Dorsal root ganglion |
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