Immunoelectron microscopic localization of carcinoembryonic antigen in gastric adenocarcinoma cell lines |
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Authors: | Kanji Ohiwa Takayuki Harada Shigeru Morikawa Teruhisa Nakamura |
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Affiliation: | Department of Surgery 2nd Unit, Shimane Medical University, lzumo, Japan;Department of Pathology 1st Unit, Shimane Medical University, lzumo, Japan |
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Abstract: | The distribution of carcinoembryonic antigen (CEA) in human gastric adenocarcinoma cell lines (HPE-GAC-3 cells and HPE-GAC-2 cells) was determined immunohistochemically by indirect peroxidase-labeled antibody method at the light and electron microscopic levels. In GAC-3 cells that proliferated as non-adherent single cells, CEA was located in the perinuclear spaces, the endoplasmic reticulum, Golgi apparatus, vesicles, multivesicular body (MVB) and entire plasma membrane. Membrane CEA was shown to be internalized into MVB in GAC-3 cells. In GAC-2 cells that form an acinus, CEA was predominantly present along the microvilli of the lumina) surface and in glycocalyceal bodies, the vesicles which bud from the microvilli into the lumen. These results suggest that in poorly differentiated cancer cells CEA is transported over the entire cell surface, retained on the membrane and accumulated Into the cell by way of the MVB, but in well differentiated cancer cells the newly synthesized CEA is rapidly and predominantly transported to the luminal surface and rapidly released from the membrane into the lumen by way of the glycocalyceal body. |
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Keywords: | carcinoembryonic antigen gastric adenocarcinoma cell line immunoelectron microscopy glycocalyceal body multivesicular body |
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