Direct inhibition of rat leydig cell function by tamoxifen |
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Authors: | T Lin EP Murono J Osterman HR Nankin |
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Institution: | 1. Medical Service, Wm. Jennings Bryan Dorn Veterans'' Hospital, Columbia, S.C. USA;2. Department of Medicine, University of South Carolina School of Medicine, Columbia, S.C. USA |
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Abstract: | The effects of tamoxifen on rat testicular steroidogenesis were studied using dispersed interstitial cells. Tamoxifen significantly inhibited LH-, and 8-bromo-adenosine 3′,5′-monophosphate (8-bromo-cyclic AMP)-stimulated testosterone synthesis in a dose-dependent manner. Tamoxifen (10?5M) also reduced LH-stimulated cyclic AMP formation. The addition of equimolar concentrations of 17β-estradiol or tamoxifen separately to interstitial cells resulted in similar inhibition of LH-stimulated testosterone synthesis. When equimolar concentrations of 17β-estradiol and tamoxifen were added concomitantly to interstitial cells, the inhibition was additive. Present studies demonstrate that tamoxifen has direct inhibitory effects on testicular steroidogenesis: both at the plasma membrane resulting in decreased cyclic AMP formation and also at steps subsequent to cyclic AMP. |
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Keywords: | Address reprint requests to Tu Lin M D Medical Service Wm Jennings Bryan Dorn Veterans' Hospital Columbia S C 29201 |
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