Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) |
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| Authors: | L. D. S. Gainey I. F. Connerton E. H. Lewis G. Turner D. J. Ballance |
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| Affiliation: | (1) Department of Microbiology, University of Reading, Whiteknights, PO Box 228, RG6 2AJ Reading, UK;(2) Department of Microbiology, University of Bristol, BS8 1TD Bristol, UK;(3) Present address: Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN Sheffield, UK;(4) Present address: Delta Biotechnology Ltd., Castle Court, Castle Boulevard, NG7 1FD Nottingham, UK;(5) Present address: Institute of Food Research, Reading Laboratory, Shinfield, RG2 9AT Reading, Berks, UK |
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| Abstract: | ![]()
Summary The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized. |
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| Keywords: | Aspergillus Neurospora Isocitrate lyase Glyoxysome Acetate metabolism |
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