顽固性偏头痛患者血清血管活性物质水平检测及临床意义分析

目的探讨顽固性偏头痛患者血清5-羟色胺(5-HT)、血管活性肠肽(VIP)、β-内啡肽(β-EP)及肿瘤坏死因子-α(TNF-α)检测水平特征及临床意义。方法选取顽固性偏头痛患者106例作为顽固性偏头痛组,110例健康成年人作为健康组,采用ELISA法检测2组人群血清5-HT、VIP、β-EP及TNF-α水平。并对顽固性偏头痛患者5-HT、VIP、β-EP及TNF-α水平进行相关性分析和多元线性回归分析。结果顽固性偏头痛患者血清5-HT、VIP、β-EP水平均低于健康组,TNF-α水平均高于健康组(P<0.05),头痛发作期患者血清5-HT、VIP、β-EP水平均低于间歇期,TNF-α水平均高于间歇期(P<0.05)。相关性分析结果显示顽固性偏头痛患者的5-HT、VIP、β-EP水平两两间呈正相关,与TNF-α水平均呈负相关(P<0.05)。多元线性回归分析显示头痛评分(分)=0.754+0.337×5-HT(μg/L)+0.216×VIP(ng/L)+0.034×β-EP(ng/L)+0.097×TNF-α(pg/mL),决定系数R 2=0.543。结论血清5-HT、VIP、β-EP及TNF-α与偏头痛存在关联,可能与顽固性偏头痛的发生与发展有关,可作为判断患者病情严重程度的潜在参考依据,但还需要今后进行更为深入的临床研究来证实。

Analysis on the concentration characteristics and clinical significance of vasoactive substances in serum for patients with intractable migraine

Objective To investigate the expression of serum 5-hydroxytryptamine(5-HT),vasoactive intestinal peptide(VIP),β-endorphin(β-EP)and tumor necrosis factorα(TNF-α)in patients with refractory migraine and analyze the clinical significance.Methods A total of 106 patients with refractory migraine were enrolled as refractory migraine group,and 110 healthy adults were as healthy group.The concentrations of serum 5-HT,VIP,β-EP and TNF-αin the two groups were detected by ELISA.The concentrations of 5-HT,VIP,β-EP and TNF-αin patients with intractable migraine were analyzed by correlation analysis and multiple linear regression analysis.Results The serum concentrations of 5-HT,VIP andβ-EP in patients with refractory migraine were lower than those in healthy group,and the concentrations of TNF-αwere higher than those in healthy group(P<0.05).The serum concentrations of 5-HT,VIP andβ-EP in patients with headache attack were lower than those in intermittent period,and the concentrations of TNF-αwere higher than those in intermittent period(P<0.05).The results of correlation analysis showed that 5-HT,VIP andβ-EP were positively correlated,and negatively correlated with TNF-αconcentrations in patients with intractable migraine(P<0.05).Multivariate linear regression analysis showed that the headache score(score)was 0.754+0.337×5-HT(μg/L)+0.216×VIP(ng/L)+0.034×β-EP(ng/L)+0.097×TNF-α(pg/mL),and the determination coefficient R 2 was 0.543.Conclusion Serum 5-HT,VIP,β-EP and TNF-αare associated with migraine,which may be related to the occurrence and development of refractory migraine.It can be used as a potential reference for judging the severity of the patient′s condition.However,more in-depth clinical research and confirmation are needed in the future.