血清miR-107在结直肠癌患者中的表达及其诊断价值分析

目的 探究血清微小RNA(miR)-107在结直肠癌患者中的表达及其对疾病的诊断价值。方法选取2016年1月至2019年1月该院收治的结直肠癌患者60例作为结直肠癌组,并选取同期在该院接受治疗的60例结直肠息肉患者作为息肉对照组,另选取同期在本院体检的健康志愿者60例作为健康对照组。采用实时荧光定量PCR法检测miR-107的相对表达量,比较3组研究对象血清癌胚抗原(CEA)、糖类抗原199(CA199)、miR-107的水平,分析血清miR-107与结直肠癌患者临床病理特征的关系,受试者操作特征曲线(ROC)分析血清miR-107、CEA、CA199对直结肠癌的诊断价值。结果 结直肠癌组的血清miR-107相对表达量以及血清CEA、CA199水平高于息肉对照组和健康对照组,差异有统计学意义(P<0.05);结直肠癌患者血清miR-107的水平与性别、年龄、肿瘤最大径无关,差异无统计学意义(P>0.05),而与分化程度、TNM分期、淋巴结转移有关,差异有统计学意义(P<0.05);miR-107所得ROC曲线下面积为0.882(95%CI:0.814~0.950),灵敏度和特异度分别为85.0%和82.9%。若和CEA及CA199进行联合应用,则灵敏度和特异度可提高至91.7%和86.7%。结论 miR-107在结直肠癌患者血清中呈高水平,而且其水平与分化程度、TNM分期、淋巴结转移有关,且对结直肠癌有较高的诊断价值。

Expression of serum miR-107 in patients with colorectal cancer and its diagnostic value for disease

Objective To investigate the expression of serum miR-107 in patients with colorectal cancer and its diagnostic value for the disease.Methods 60 patients with colorectal cancer admitted to the hospital from January 2016 to January 2019 were enrolled as colorectal cancer group.60 patients with colorectal polyps treated in the hospital were selected as the polyp control group.60 healthy volunteers in the hospital were used as healthy controls.The relative expression of miR-107 was detected by real-time fluorescent quantitative PCR.The expressions of carcinoembryonic antigen(CEA),carbohydrate antigen 199(CA199)and miR-107 were compared between the three groups.The relationship between serum miR-107 and clinicopathological features of colorectal cancer patients was analyzed.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum miR-107,CEA and CA199 for colorectal cancer.Results The relative expressions of serum miR-107 and serum CEA and CA199 levels in colorectal cancer group were higher than those in polyp control group and healthy control group,and the differences were statistically significant(P<0.05).The expression of serum miR-107 in colorectal cancer patients was not related to gender,age and tumor diameter(P>0.05),but was related to differentiation degree,TNM stage and lymph node metastasis(P<0.05).The area under the ROC curve obtained by miR-107 was 0.882(95%CI:0.814-0.950),and the sensitivity and specificity were 85.0%and 82.9%,respectively.When combined with CEA and CA199,the sensitivity and specificity can be increased to 91.7%and 86.7%.Conclusion miR-107 is highly expressed in the serum of patients with colorectal cancer.The expression level is related to the degree of differentiation,TNM stage and lymph node metastasis,and it has a high diagnostic value for colorectal cancer.