非洲爪蟾VEGF肿瘤疫苗治疗小鼠纤维肉瘤的研究

目的：探讨非洲爪蟾VEGF肿瘤疫苗免疫抗小鼠纤维肉瘤的作用.方法：采用BALB/c小鼠建立Meth A小鼠纤维肉瘤模型.实验肿瘤小鼠随机分成3个治疗组：非洲爪蟾VEGF（xVEGF）组、小鼠VEGF（mVEGF）组、生理盐水对照（NS）组.观察小鼠肿瘤生长、生存率和治疗的毒副反应,并检测抗自身mVEGF抗体、肿瘤微血管密度（microvessel density, MVD）.结果：xVEGF组肿瘤明显小于mVEGF组或NS对照组（P＜0.05）,xVEGF组小鼠生存率也明显高于mVEGF组或NS对照组（P＜0.01）.Western Blot检测发现异种蛋白质疫苗免疫的小鼠体内产生了抗自身mVEGF抗体,xVEGF组肿瘤MVD明显低于mVEGF组或NS对照组（P＜0.01）.结论：非洲爪蟾VEGF疫苗治疗可诱导纤维肉瘤小鼠产生抗自身VEGF免疫反应及抗肿瘤作用.

Antitumor Effect of Xenopus Laevis VEGF Vaccine on Fibrosarcoma in Mice

Objective:To explore antitumor effect of immunotherapy with recombinant Xenopus laevis vascular endothelial growth factor(xVEGF)as a xenogeneic vaccine on fibrosarcoma in mice.Methods:Meth A fibrosarcoma model was established in BALB/c mice.These mice were randomly divided into three groups:xVEGF,mVEGF(mouse VEGF)and normal saline(NS)groups,and received relative treatments.Tumor growth,survival rate of tumor-bearing mice,and potential toxicity of the vaccines were investigated.Anti-mVEGF antibodies were detected by Western blot analysis.In addition,microvessel density(MVD)of tumor was detected by immunohistochemistry.Results:Tumor volumes of mice were significantly smaller in xVEGF group than in mVEGF or NS group(P<005),and survival rate of mice was also significantly higher in xVEGF group than in mVEGF or NS group(P<001).Anti-mVEGF antibodies were found by Western blot analysis in mice treated with xVEGF vaccine.Moreover, MVD in tumor tissues was significantly lower in xVEGF group than in mVEGF or NS group(P<001).Conclusion:Immunotherapy with xVEGF as a xenogeneic vaccine induces antitumor effect in mice by inducing immune response against self-VEGF.