音乐电针和脉冲电针镇痛作用的对比性研究

目的:通过音乐电针与脉冲电针在行为学和神经电生理学两个方面镇痛作用的对比观察,证实音乐电针镇痛作用的优越性。方法:将大鼠分为脉冲电针组、音乐电针组和空白对照组,采用热甩尾反射和尾核、海马痛兴奋性和痛抑制性神经元细胞放电技术作为检测指标,探讨脉冲电针和音乐电针对腧穴兴奋性的调节,分析两种电针镇痛作用的效果。结果:音乐电针的镇痛作用明显优于脉冲电针。脉冲电针多次应用后,针刺效应作用出现逐渐减弱现象,表明机体穴位产生了电针耐受作用,而音乐电针则未出现针刺耐受。结论:音乐电针疗法具有克服电针耐受作用,从而能更好地发挥穴位功能。

Comparative Research on the Analgesic Effect Induced by Music Electroacupuncture and Pulse Electroacupuncture

Objective: To prove the priority of music electroacupuncture (EA) in inducing analgesia by comparing its effect with that of pulse EA from behavior and neuroelectrophysiology. Methods: 120 Wistar rats anesthetized with 5% urethane + 0.5% chloralose (i.p., 10 mL/kg) were randomized into behavior part and neuronal reaction observation which were subdivided into control group, music-EA group and pulse-EA group respectively with 20 cases in each group. Pain reaction to radiation-heat irradiation was observed by using tail flick latency (TFL) test. Electric pulse EA and music (music sound waves) EA (6 Hz, 2 V and a suitable strength able to induce local muscular tremor) were applied to bilateral “Zusanli”(ST 36) and “Sanyinjiao”(SP 6) for 30 min/time, 6 times altogether, followed by detecting TFL. Extracellular discharges of neurons of the left caudate nucleus (CN) and the right hippocampus (HIP) were recorded by using glass micropipettes. Reactions of neuronal firing to electric stimulation of sciatic nerve (5 mA, duration of waves 0.3 ms, interval 5 ms, 5 pulses/train) or EA were fed to Power-Lab/8s data processing system. Results: In comparison with control group and pre-EA, TFL values of pulse EA from the 1 st to 3 rd time of EA, and those of music EA from the 1 st to 6 th time of EA delayed significantly (P<0.05～0.01). TFL values of music EA group were significantly bigger than those of pulse EA and control groups from the 1 st to 6 th time of EA (P< 0.05～0.01 ). Compared with the latency values of pain excitement reaction of firings of CN and HIP neurons of control group, those of both pulse EA and music EA groups increased significantly (P<0.05), while the net increase values of the firing frequency of the two EA groups were markedly smaller (P<0.05～0.01); and the effects of music EA were significantly better than those of pulse EA from the 1 st time of EA on (P< 0.05～0.01 ). The effects of music EA and pulse EA on pain inhibitory reaction of neuronal firings were similar to those of pain excitement neurons. Results showed that the analgesic effect of music EA was significantly superior to that of pulse EA and the analgesic effect of pulse EA was weakened gradually after multiple times of EA stimulation. Conclusion: Music EA has a better analgesic effect and has no marked tolerance phenomenon which may favor functions of acupoint stimulation.