全反式维甲酸对翼状胬肉成纤维细胞增殖的影响

目的:观察全反式维甲酸(ATRA)对体外培养人翼状胬肉成纤维细胞(HPF)增殖的影响,寻找治疗和预防翼状胬肉复发的新途径。方法:体外培养翼状胬肉患者成纤维细胞;将不同浓度的ATRA作用于成纤维细胞分别达24,48h和72h后,MTT法检测ATRA对HPF的影响,免疫组织化学染色增生细胞核抗原(PCNA)检测细胞生长活性。结果:MTT法显示10-8mmol/LATRA作用24h后能抑制HPF的增殖(P<0.05),呈剂量和时间依赖性。ATRA能浓度依赖性地抑制细胞表达PCNA(P<0.05)。结论:ATRA能够显著抑制HPF的增殖。

Effect of all trans-retinoic acid on the proliferation of human pterygium fibroblasts

AIM: To investigate the effect of all trans- retinoic acid(ATRA)on proliferation of human pterygium fibroblasts(HPF)in vitro and search for a new method to prevent the recurrence after pterygium surgery.

METHODS: HPF was cultivated in vitro. MTT assay was used to detect the effect on proliferation of HPF after incubation for 24 hours, 48 hours and 72 hours in the different concentration of ATRA. The expression of proliferating cell nuclear antigen(PCNA)in each group was detected by immunohistochemical staining.

RESULTS: Administration of 10^-8 mmol/L ATRA for 24 hours could significantly inhibit HPF proliferation in a dose- and-time dependent manner(P<0.05). ATRA could inhibit the expression of PCNA in HPF in a dose-dependent manner(P<0.05).

CONCLUSION:ATRA can significantly inhibit HPF proliferation.