胰岛素样生长因子ⅡmRNA结合蛋白3对胆囊癌迁移侵袭的影响

目的探究胰岛素样生长因子Ⅱ(insulin-like growth factorⅡ,IGF-Ⅱ)mRNA结合蛋白3(IGF2BP3)对胆囊癌迁移侵袭的作用。方法应用实时定量PCR(qRT-PCR)法检测23份胆囊癌组织和23份癌旁组织中IGF2BP3的表达水平;在胆囊癌细胞中利用小干扰RNA(siRNA)敲减目的基因IGF2BP3;Transwell迁移实验与带胶Transwell侵袭实验检测敲减IGF2BP3后对胆囊癌细胞迁移侵袭能力的影响;细胞免疫荧光法检测敲减IGF2BP3后上皮细胞间充质转化(epithelial mesenchymal transition,EMT)相关蛋白表达水平变化。结果 IGF2BP3在胆囊癌组织中高表达(P0.05);与正常胆管上皮细胞比较,5株胆囊癌细胞系中IGF2BP3高表达;敲减IGF2BP3后,胆囊癌细胞系的迁移侵袭能力明显降低(P0.0001);敲减IGF2BP3后EMT相关蛋白表达水平下调。结论 IGF2BP3通过促进EMT,从而促进胆囊癌侵袭转移。

Effect of insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) on migration and invasion of gallbladder cancer

Objective To investigate the effect of insulin-like growth factor Ⅱ mRNA binding protein 3 (IGF2BP3) on migration and invasion of gallbladder carcinoma.Methods The expression of IGF2BP3 in gallbladder carcinoma and adjacent tissues was detected by real-time quantitative PCR (qRT-PCR).As compared with the normal bile duct epithelial cells,the expression of IGF2BP3 in 5 strains of gallbladder cancer cell lines was significantly up-regulated.The effect of IGF2BP3 on migra tion and invasion of gallbladder carcinoma cells was detected by Transwell migration assays.Immunofluorescence assay was used to detect the expression of the epithelial mesenchymal transition (EMT)-related proteins.Results The relative expression level of IGF2BP3 was significantly higher in tumor tissues than in their non-cancerous counterparts.After knocking down the expression of IGF2BP3,the migration and invasion ability in 5 strains of gallbladder carcinoma cell lines was significantly reduced.The expression level of EMT-related proteins was down-regulated after knocking down the expression of IGF2BP3.Conclusions IGF2BP3 promotes the migration and invasion of gallbladder carcinoma by induction of EMT.