流式细胞术建立甘肃地区学龄前汉族儿童外周血淋巴细胞亚群相对计数的正常参考值范围

目的 建立甘肃地区学龄前汉族儿童外周血淋巴细胞亚群相对计数的正常参考值范围。方法 纳入2018年1月31日至2019年1月30日在甘肃省妇幼保健院(我院)行鞘膜积液、疝、血管瘤等手术或常规体检的0~7岁汉族健康儿童,排除患心脑血管疾病、血常规或肝肾功能异常、行淋巴细胞亚群检测前3周内存在感染、有先天性免疫缺陷疾病者。分为新生儿组、婴儿组、幼儿组和学龄前组。均采集静脉血2 mL(EDTA抗凝),于24 h内用四色荧光标记流式细胞术完成淋巴细胞亚群检测,以(P_2.5,P_97.5)作为各指标的正常参考值范围。结果 ①共792名儿童进入本文分析,男472名,女320名,新生儿组174名、婴儿组216名、幼儿组170名、学龄前组232名。②不同性别之间比较,新生儿组CD4⁺T细胞、CD8⁺T细胞、B细胞、NK细胞百分比和CD4⁺/CD8⁺比值差异有统计学意义,婴儿组CD4⁺T细胞、CD8⁺T细胞、B细胞百分比和CD4⁺/CD8⁺比值差异有统计学意义,学龄前组T细胞、CD4⁺T细胞、B细胞、NK细胞百分比差异有统计学意义。③男、女各年龄组组间整体比较,淋巴细胞亚群百分比差异均有统计学意义。T细胞、CD4⁺T细胞百分比随年龄增长呈降低趋势,男女童降低趋势相近;CD8⁺T细胞百分比随年龄增长呈升高趋势,女童增高趋势更明显;B细胞百分比在1岁前呈上升趋势,后呈降低趋势,女童的变化趋势较为缓和;NK细胞的变化与B细胞相反,在1岁内降低,而后升高,男童变化趋势更明显。结论 健康儿童淋巴细胞亚群的分布与地域、年龄段和性别有关。

The establishment of reference ranges of peripheral blood lymphocyte subpopulations for Gansu Han children of preschool age based on flow cytometry

Objective To establish the reference ranges of peripheral blood lymphocyte subpopulations values in Gansu children of preschool age.Methods Gansu Han children who received a preoperative, postoperative or routine physical examination aged from 0 to 7 years old were enrolled into the study. The children, who have cardio-cerebral vascular disease, abnormal blood routine, liver and kidney dysfunction, infection within 3 weeks of lymphocyte subpopulation detection, primary immunodeficiency disease, were excluded. Included children were divided into neonatal group (0 to 28 d), infant group (28 d to 12 months), toddler age group (1 to 3 years) and preschool age group (3 to 7 years). Peripheral blood was collected, and relative counts of lymphocyte subpopulations were detected by flow four, color cytometric analysis within 24 h. The (P_2.5, P_97.5) was used as the normal reference range of each index.Results ①A total of 792 children were enrolled into the analysis of this article, including 472 boys, 320 girls, 174 newborns, 216 infants, 170 toddlers, and 232 preschoolers. ②The interior group comparison between males and females showed there was a statistically significant difference between males and females in CD4⁺T、CD8⁺T、B、NK lymphocyte percentages and CD4⁺/CD8⁺ value in the neonatal group; in CD4⁺T、CD8⁺T、B lymphocyte percentages and CD4⁺/CD8⁺ value in the infant group; in T、CD4⁺T、B、NK lymphocyte percentages in the preschool age group. ③The percentages of lymphocyte subpopulations were statistically significant in the distribution of girls and boys in all age groups. The T and CD4⁺T lymphocyte percentages declined with age, and the trend in decreasing degree was similar between boys and girls. The CD8⁺T lymphocyte percentage increased with age, and the trend of girls in increasing degree was more obvious. B lymphocyte percentage showed a marked rise in children within 1 year old (from neonatal group to infant group), followed by a gradual decline, and the trend of girls was more moderate. NK lymphocyte percentage, on the other hand, displayed a pattern of change almost opposite that for B lymphocyte,which decreased within 1 year and then increased, and the trend of boys was more obvious.Conclusion The distribution of lymphocyte subpopulations percentages in healthy children is related to region, age and gender.