急性颅脑损伤脑脊液中兴奋性氨基酸含量变化的临床分析

为研究急性颅脑损伤过程中脑脊液中兴奋性氨基酸含量的变化 ,对实验组 (n =3 0例 ,按格拉斯哥昏迷分级计分分成轻型、中型、重型 3组 )和对照组 (n =1 0例 ) ,于颅脑外伤后 0～ 48h经腰椎穿刺采集脑脊液 ,用氨基酸自动分析仪 ,采用液相层析测定其结果。结果显示轻型、中型、重型 3组在急性颅脑损伤后兴奋性氨基酸含量均较对照组有所增加 ,且升高幅度依次递增。提示 :急性颅脑损伤后脑内兴奋性氨基酸的浓度变化与颅脑损伤的程度有关 ,损伤越重 ,升高越明显

Clinical Analysis of Excitatory Amino Acids Level in Cerebrospinal Fluid after Acute Brain Injury

In order to study the neurobiochemical changes and excitotoxicity to neural cells of the excitatory amino acids (EAAs) in cerebrospinal fluid (CSF) after acute brain injury, a total of 30 patients were classified into 3 groups according to the score of Glasgow Coma Scale (GCS): mild, moderate and severe groups, while the other 10 patients were observed as the controls. Fresh CSF was sampled by lumbar puncture no later than 48 h after brain injury in all cases. Concentrations of the EAAs were measured by high performance liquid chromatography (HPLC). The result showed that the mean concentration of EAAs in CSF of severe, moderate and mild groups were significantly higher in order than that of the controls. The result indicates that the implication of this study is that acute brain injured patrents are exposed to a high concentrations of EAAs. These abnormalities are related to the severity of injury.