| 丹参酮ⅡA诱导NB4细胞凋亡时Caspase3活性变化 |
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| 引用本文： | 梁效功,羊裔明,邓承祺,孟文彤,刘霆,贾永前,程韵枫,李力. 丹参酮ⅡA诱导NB4细胞凋亡时Caspase3活性变化[J]. 四川大学学报(医学版), 2003, 34(3): 549-551 |
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| 作者姓名： | 梁效功 羊裔明 邓承祺 孟文彤 刘霆 贾永前 程韵枫 李力 |
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| 作者单位： | 四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041;四川大学华西医院,血液科,成都,610041 |
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| 摘 要： | 目的：研究Caspase3在丹参酮ⅡA(TanⅡA)诱导NB4细胞凋亡过程中的变化及与凋亡的关系。方法：通过细胞形态学、DNA含量分析及DNA片段比率,证实TanⅡA是否可诱导NB4细胞凋亡,用荧光分光光度仪检测Caspase3活性及N-乙酰-天冬氨酸-谷氨酸-颉氨酸-天冬氨酸-7氨基-4甲基-香豆素(AC-DEVD-AMC)行Caspase3抑制试验探讨细胞凋亡与Caspase3间的关系。结果：TanⅡA可诱导NB4细胞凋亡,并伴有Caspase3活性增高。AC-DEVD-乙醛(AC-DEVD-CHO)可抑制TanⅡA诱导NB4细胞凋亡。结论。TanⅡA诱导NB4细胞凋亡可通过激活Caspase3而实现。
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| 关 键 词： | 丹参酮ⅡA NB4细胞 凋亡 Caspase3活性 |
| 修稿时间： | 2002-05-20 |
The Variation of Caspase3 Activity in Tanshinone Induced NB4 Cells Apoptosis |
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| Liang Xiaogong,Yang Yiming,Deng Chengqi,Meng Wentong,Liu Ting,Jia Yongqian,Cheng Yunfeng,Li Li. The Variation of Caspase3 Activity in Tanshinone Induced NB4 Cells Apoptosis[J]. Journal of Sichuan University. Medical science edition, 2003, 34(3): 549-551 |
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| Authors: | Liang Xiaogong Yang Yiming Deng Chengqi Meng Wentong Liu Ting Jia Yongqian Cheng Yunfeng Li Li |
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| Affiliation: | Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To study the variation and role of Caspase3 activity in the process of Tanshinone (Tan II A) induced NB4 cells apoptosis. METHODS: NB4 cell apoptosis induced by Tan II A was demonstrated by cell morphology, DNA content analysis and DNA fragmentation assay. Caspase3 activity was determined by spectrofluorometry, and its inhibitory assay was performed using N-acetyl-Asp-Glu-Val-Asp-aldehyde(AC-DEVD-CHO). RESULTS: Tan II A could induce NB4 cell apoptosis accompanied with increase of caspase3 activity. The induction of NB4 cell apoptosis by use of Tan II A could be partially inhibited by AC-DEVD-CHO. CONCLUSION: The induction of NB4 cell apoptosis by Tan II A could be fulfilled by activating Caspase3. |
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