数字减影血管造影技术在犬下肢动脉造影中的应用

文题释义：数字减影血管造影步进技术：在只注射一次对比剂的情况下,根据不同血流速度和充盈程度,通过控制造影床的移动,进行跟踪造影,一次注射对比剂即可实现全部血管显影;相比于传统的分段造影,可显著减少对比剂用量及曝光时间。动物模型：通过对实验动物进行特定的外部干预,使动物产生某一方面的疾病状态或生理变化,达到对人体特定情况的模拟,从而以此为平台进行反复的观察和研究,以便更准确地观察模型的实验结果并与人类疾病进行比较研究,有助于更方便、更有效地认识人类疾病的发生发展规律,研究防治措施。背景：目前对下肢血管疾病的研究趋于热门,在以大型动物为研究平台的实验中,需要对动物下肢血管进行形态及密度的检测,以研究和验证干预措施的有效性,且这种检测方法需要较高的可行性及可重复性。目的：探讨数字减影血管造影技术应用于犬类动物模型下肢动脉造影中的可行性、影响因素及应用价值。方法：6只比格犬经麻醉后固定于工作床,于犬大腿上段股动脉搏动明显处,采用留置针进行经皮股动脉顺行穿刺,人工推注对比剂,采用数字减影血管造影步进技术采集造影图像,对图像效果及影响因素进行分析。结果与结论：①所有6只比格犬双后肢最终动脉造影图像显示清晰,能分辨细小血管分支,无伪影;②其中第1只犬在初次造影时因对比剂刺激导致肢体抖动,图像效果欠佳,经固定肢体及稀释对比剂后再次造影得到了清晰图像;③提示采用数字减影血管造影技术进行犬类动物模型动脉造影可以清晰显示血管形态及密度,操作简单、重复性好,对模拟肢体缺血的动物模型研究具有重要意义。ORCID: 0000-0003-0335-021X(张世勇)中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程

Application of digital subtraction angiography in dog hindlimb arteriography

BACKGROUND:The research on lower extremity vascular disease is becoming popular.In the experiment of large animals as research platform,it is necessary to detect the shape and density of lower extremity vascular in order to study and verify the effectiveness of intervention measures,and this detection method needs high feasibility and repeatability.OBJECTIVE:To investigate the feasibility,influencing factors and application value of digital subtraction angiography in lower limb arteriography of a canine animal model.METHODS:Six Beagle dogs were fixed on the working bed after anesthesia and punctured percutaneously through the femoral artery with an indwelling needle.Contrast agent was injected artificially.Digital subtraction angiography bolus chase technology was used to collect contrast images.The image effect and influencing factors were analyzed.RESULTS AND CONCLUSION:(1)The final arteriography images of all six Beagle dogs’hind limbs were clear,and the branches of small vessels could be distinguished without artifacts.(2)The first beagle shook when encountering contrast agent stimulation during the initial angiography,resulting in poor image effect.After fixing the limbs and diluting the contrast agent,the clear images were obtained by re-angiography.(3)These results indicate that arteriography using digital subtraction angiography can clearly reveal the shape and density of blood vessels in canine animal models.It is simple and reproducible,and is of great significance for research on animal models of limb ischemia.