松花粉对甘油诱导大鼠急性肾衰竭的保护作用

目的：研究松花粉对甘油诱发大鼠急性肾衰竭（acute renal failure，ARF）的保护作用及机制。方法：采用双侧后肢注射50%甘油溶液（10 mL·kg-1）诱发大鼠成急性肾衰竭模型，检测大鼠血清肌酐（Scr）、血清尿素氮（BUN）的含量，肾组织中超氧化物歧化酶（SOD）的活性，谷胱甘肽（GSH）、丙二醛（MDA）的含量，同时测定肾组织中一氧化氮（NO）的含量和诱导型一氧化氮合酶（iNOS）的活性；HE染色观察肾病理组织形态学变化。结果：与空白组大鼠相比，模型组大鼠血清Scr和BUN的含量明显上升，肾组织中SOD的活性和GSH的含量显著降低，MDA的含量显著升高；组织病理检查发现，模型组大鼠肾小管损伤明显，肾皮质细胞部分脱落，肾小球肿胀，间质炎性侵润明显。给予松花粉治疗后，可显著降低ARF大鼠血清Scr和BUN，升高肾组织中SOD的活性、GSH的含量，降低MDA的含量，同时发现，松花粉可明显降低肾组织中NO的含量和iNOS的活性，并显著降低肾组织损伤。结论：松花粉对甘油致大鼠ARF具有治疗作用，其作用机制可能与抑制iNOS的活性，降低体内NO过多产生，降低NO相关的脂质过氧化过程有关。

Protective effects of pollen pini against glycerol-induced acute renal failure in rats

Objective: To study the protective effect of pollen pini against acute renal failure induced by glycerol and its underlying mechanisms in rats. Methods: The rats were intramuscularly injected with 50% glycerol (10 mL/kg) in each hind leg. After the pini treatment for seven days, the kidney damage was established. The content of serum creatinine (Scr) and urea nitrogen (BUN), the activties superoxide dismutase (SOD), inducible nitric oxide synthase (iNOS), and the content of glutathione (GSH) and Malondialdehyde (MDA) and NO in the kidney tissue were examined. Results: Campared with the control group rats, the the content of Scr and BUN in serum, the content of MDA and NO, the activities of iNOS in kiney tissue were increased, the activities of SOD and the content of GSH were decreased. And the sections show the kiney tissue damage was obviously, the glomerular volume became bigger, swelling and degeneration of endothelial cells, renal interstitial inflammatory cell infiltration. However, pollen pini dose-dependently exhibited significant decrease in the content of Scr and BUN in the plasma, reduced the content of MDA and NO, and decreased the activities if iNOS in the kidney tissue. Also, it increased the activities of SOD, and the content of GSH in the kidney tissue. The damage decreased after the administration of pollen pini. Conclusion: The results indicated that pollen pini had a protective effect against glycerol-induced ARF in rats. It is considered that this protective effect may be related to its activities inhibition of iNOS and the level of NO, and its free radical scavenging related to NO.