在体骨髓原位造血细胞间纳米通道的扫描电子显微镜(SEM)证据

 目的  探讨在体骨髓细胞之间是否存在细胞间纳米通道(intercellular nanotubes)。方法  利用扫描电子显微镜(scanning electron microscope,SEM)在体原位观察C57BL/6小鼠骨髓中细胞间纳米通道的分布、形态以及可能的形成机制。结果  骨髓造血细胞之间存在纳米通道结构。SEM显示该结构在骨髓组织中散在分布,位于骨髓内血窦内、外侧。骨髓造血细胞间纳米通道的管径粗细不均,平均长度为5.85 μm (1.58~18.54 μm),平均直径为364 nm (202~541 nm),还可见一些小颗粒状物质黏附在纳米通道表面。此外,小鼠骨髓造血细胞可能通过伸出突起的形式形成细胞间纳米通道。结论  本研究首次为小鼠骨髓造血细胞之间存在细胞间纳米通道提供了形态学证据。

Intercellular nanotube between hematopoietic cells in bone marrow: scanning electron microscope (SEM) evidence in vivo

Objective  To investigate whether intercellular nanotubes are present in bone marrow in vivo. Methods  Scanning electron microscopy (SEM) was applied to observe the distribution and morphology and proposed formed mechanism of intercellular membrane nanotubes in C57BL/6 mice bone marrow in situ. Results  The results revealed that intercellular nanotubes emerged in a pattern of scattered state in the entire bone marrow, especially inside and/or outside sinusoid. Moreover, the intercellular nanotubes with uneven diameters were shown 5.85 μm in average length (1.58-18.54 μm) and 364 nm in average width (202-541 nm), as well as clusters of small granules adhered to the surface of intercellular nanotubes. In addition, proposed mechanisms of the formation of intercellular nanotubes in mice bone marrow were elucidated that membrane continuity was formed by free-ended protrusions. Conclusions  The present study for the first time captured the visual evidence for the existence of intercellular nanotubes in mice bone marrow in vivo.