吻内侧被盖核(RMTg)参与吗啡引起的大鼠睡眠紊乱

 目的 旨在阐明吻内侧被盖核(rostromedial tegmental nucleus,RMTg)是否参与吗啡引起的大鼠睡眠障碍。方法 将雄性SD大鼠随机分为溶剂对照组和吗啡组,每组7只,对照溶剂为人工脑脊液(artificial cerebrospinal fluid,ACSF)。采用脑立体定位、核团微量注射和睡眠记录与解析等技术观察RMTg内给予吗啡对大鼠睡眠-觉醒周期的影响。结果 与对照组相比,双侧RMTg 给予吗啡(16 mmol/L,每侧0.5 μL)可以引起大鼠长达4 h的觉醒,期间非快动眼(non-rapid eye movement,NREM)睡眠深度降低、快动眼(REM)睡眠减少的现象与吗啡临床用药所引起的睡眠障碍的表现相一致。结论 RMTg参与吗啡引起的大鼠睡眠紊乱。

The rostromedial tegmental nucleus (RMTg) participates in sleep disruption caused by morphine in rats

Objective  To clarify the potential role of rostromedial tegmental nucleus (RMTg) in morphine-caused sleep disruption in rats. Methods  Male SD rats were randomly divided into vehicle control group (n=7) treated with artificial cerebrospinal fluid (ACSF) and morphine group (n=7). By using brain stereotaxic, nucleus microinjection and sleep recording techniques,we observed the effects of microinjection of morphine into rat RMTg on the sleep-wake cycle. Results  Compared with the control group, microinjection of morphine (16 mmol/L, 0.5 μL perside) into the bilateral RMTgs induced 4-hour wakefulness. During this period, the depth of non-rapid eye movement (NREM) sleep became light and the amount of rapid eye movement sleep decreased, which conformed to the phenotype of sleep disruption caused by clinical morphine usage. Conclusions  The RMTg may be involved in the sleep disruption caused by morphine in rats.