乌司他丁对促炎症因子诱导的肺微血管内皮细胞通透性和细胞因子产生的影响

目的:研究乌司他丁对炎症因子诱导的体外培养的大鼠肺微血管内皮细胞通透性和细胞因子产生的影响.方法:体外培养大鼠肺微血管内皮细胞分组进行实验:①对照组:内皮细胞培养液中加入生理盐水或乌司他丁;②炎症因子刺激组:因培养液中分别含烫伤血清、胰蛋白酶、前列腺素E2(PGE2)、缓激肽、组胺而分为5个炎症因子刺激亚组;③乌司他丁...

Effects of ulinastatin on permeability of pulmonary microvascular endothelium and production of pro-inflammatory cytokines induced by inflammatory factor

Objective： To investigate the effects of ulinastatin on changes in microvascular endothelial permeability and cytokine production induced by inflammatory factor. Methods：The cultured pulmonary microvascular endothelial cells （PMECs） were divided into 3 groups：①Control group： Normal saline or ulinastatin was added into cell culture. ② Inflammatory factors-stimulating group： It was redivided into five subgroups in which scald serum, trypsin, bradykinin, histamine or prostaglandin F4 （PGE2）was added respectively into PMECs culture. ③ Ulinastatin treatment group： It was redivided into five subgroups in which ulinastatin was added separately into PMECs culture in which inflammatory factors were respectively added as inflammatory factors-stimulating group. The permeability of monolayer PMECs was determined by the method of Transwell. The production of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） was determined by enzyme-linked immunoadsorbent assay （ELISA）. Results：The permeability of monolayer PMECs, and the production of TNF-a and IL-6 in each inflammatory factor-stimulating group were significantly increased compared with those of control group （P〈0. 01）. The permeability of PMECs, and the production of pro-inflammatory factors in scald serum and trypsin groups were markedly lowered after ulinastatin treatment compared with those groups without ulinastatin treatment （all P 〈0. 01）, while the levels of bradykinin, histamine and PGF4 groups were not lowered compared with non-ulinastatin treatment groups（P〈0. 05）. Conclusions：The results indicate that ulinastatin significantly inhibit the increase in the permeability of PMECs and the production of TNF-α and IL-6 induced by the scald serum and trypsin,while it has no significant effect on the increased permeability of PMECs and pro-inflammatory factors released from PMECs after treatment with bradykinin, histamine and PGF4.