毛冬青甲素对糖尿病肾病大鼠肾小球保护作用实验研究

目的:研究毛冬青甲素对糖尿病肾病大鼠模型的肾小球纤维化的影响,探讨毛冬青甲素抗肾小球纤维化的作用机制。方法:大鼠48只随机分为治疗组和对照组。建立链脲佐菌素诱导的糖尿病肾病大鼠模型后,治疗组:腹腔注射毛冬青甲素40 mg/kg;对照组:腹腔注射生理盐水0.5 mL。0天、7天、14天、28天分别处死,各组取6只大鼠。免疫组织化学法测定α-平滑肌肌动蛋白(α-SMA)、单核细胞趋化蛋白-1(MCP-1)的表达。结果:2组大鼠的收缩压在14天、28天比较,差异均有非常显著性意义(P0.01);治疗组在第7、14、28天的血肌酐、血尿素氮、24 h尿白蛋白均低于对照组,差异均有非常显著性意义(P0.01)。在第7、14、28天治疗组和对照组的MCP-1表达均差异显著(P0.01);治疗组第7、14、28天的α-SMA表达均低于对照组,差异均有非常显著性意义(P0.01)。结论:毛冬青甲素可通过降低α-SMA、MCP-1,抑制单核/巨噬细胞浸润和和系膜细胞增生,减轻肾小球纤维化。

Experimental Study of Ilexonin A in Preventing Renal Glomerulus of Diabetic Nephropa- thy Rats

Objective. To investigate the effect of ilexonin A on the fibrosis of renal glomerulus of STZ-induced diabetic nephrypathy rats, and to explore its possible mechanism of anti-renal-fibrosis. Methods： Forty-eight male Wistar rats were randomized into control group and ilexonin A group. Six rats of each group were killed respectively on day 0, 7, 14, 28 after the establishment of STZ-induced diabetic nephrypathy model. Immunohistochemistry was performed to measure the expression levels of α-smooth muscle actin（α-SMA） and monocyte chemoattractant protein-1 （MCP-1）. Results- Qn day 14 and 28, the difference of systolic pressure was significant between the two groups （P 〈 0.01）. The contents of serum creatinine, blood urea nitrogen and urinary albumin as well as the expression levels of α-SMA and MCP-1 in ilexonin A group were lower than those in the control group on day 7, 14, and 28 （P 〈 0.01）. Conclusion. Ilexonin A can inhibit renal fibrosis by decreasing the expression levels of α-SMA and MCP-1, and by relieving monocyte/macrophage infiltration and mesangial cell proliferation.