人透明软骨抗OS-732细胞系浸润的研究——细胞培养及透射电镜观察

本文研究成骨肉瘤OS-732细胞系浸润软骨的机理,指出经盐酸胍处理后的软骨可被瘤细胞所浸润,这些浸润细胞在电镜下见其有生长、代谢旺盛的特点,提示正常软骨内含有抑制肿瘤细胞浸润的因子存在。

Human Hyaline Cartilage Inhibition of OS-732 Cell Line Invasion in vitro

The anti-invasive ability of cartilage was investigated in human hyaline cartilage co-cultured with OS-732 human osteosarcoma cell line. Invasive osteosarcoma cells were unable to invade normal hyaline cartilage after 7-day co-culture. Observation of TEM showed that the growth and metabolism of tumor cells were inhibited by co-cultured cartilage. However, cartilage extracted with 5M GuHCl was invaded by tumor cells. In this situation, tumor cells indicated a high growth and metabolic status and contained abundant microtubules, microfilaments, rough endoplasmic reticulum, mitochondria and Golgi bodies. Cartilage matrix collagenous protein(CMCP), from extracted cartilage, was denatured and became more electron dense. In the control group, normal fibroblasts did not invade cartilage or extracted cartilage and its growth and metabolism did not inhibit by cartilage. Our report shows that resistance of hyaline cartilage to tumor invasion could be regulated in part by salt-derived materials which may inhibit the growth, metabolism and locomotion of tumor cells.